1n33: Difference between revisions
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'''Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin''' | '''Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin''' | ||
| Line 30: | Line 27: | ||
[[Category: Tarry, M J.]] | [[Category: Tarry, M J.]] | ||
[[Category: 30s ribosomal subunit]] | [[Category: 30s ribosomal subunit]] | ||
[[Category: | [[Category: A site]] | ||
[[Category: | [[Category: Antibiotic]] | ||
[[Category: | [[Category: Anticodon]] | ||
[[Category: | [[Category: Codon]] | ||
[[Category: | [[Category: Decoding]] | ||
[[Category: | [[Category: G:u]] | ||
[[Category: | [[Category: Gu]] | ||
[[Category: | [[Category: Messenger rna]] | ||
[[Category: | [[Category: Mismatch]] | ||
[[Category: | [[Category: Mrna]] | ||
[[Category: | [[Category: Near-cognate]] | ||
[[Category: | [[Category: Paromomycin]] | ||
[[Category: | [[Category: Ribosome]] | ||
[[Category: | [[Category: Stem-loop]] | ||
[[Category: | [[Category: Transfer rna]] | ||
[[Category: | [[Category: Trna]] | ||
[[Category: | [[Category: Wobble]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 02:01:48 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 02:01, 3 May 2008
Structure of the Thermus thermophilus 30S ribosomal subunit bound to codon and near-cognate transfer rna anticodon stem-loop mismatched at the second codon position at the a site with paromomycin
OverviewOverview
A structural and mechanistic explanation for the selection of tRNAs by the ribosome has been elusive. Here, we report crystal structures of the 30S ribosomal subunit with codon and near-cognate tRNA anticodon stem loops bound at the decoding center and compare affinities of equivalent complexes in solution. In ribosomal interactions with near-cognate tRNA, deviation from Watson-Crick geometry results in uncompensated desolvation of hydrogen-bonding partners at the codon-anticodon minor groove. As a result, the transition to a closed form of the 30S induced by cognate tRNA is unfavorable for near-cognate tRNA unless paromomycin induces part of the rearrangement. We conclude that stabilization of a closed 30S conformation is required for tRNA selection, and thereby structurally rationalize much previous data on translational fidelity.
About this StructureAbout this Structure
1N33 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Selection of tRNA by the ribosome requires a transition from an open to a closed form., Ogle JM, Murphy FV, Tarry MJ, Ramakrishnan V, Cell. 2002 Nov 27;111(5):721-32. PMID:12464183 Page seeded by OCA on Sat May 3 02:01:48 2008