1n2n: Difference between revisions

Revision as of 02:00, 3 May 2008

Crystal structure of cyanide complex of the oxygenase domain of inducible nitric oxide synthase.

OverviewOverview

The crystal structure of the ternary cyanide complex of P450cam and camphor was determined to 1.8A resolution and found to be identical with the structure of the active oxygen complex [I. Schlichting et al., 2000, Science 287, 1615]. Notably, cyanide binds in a bent mode and induces the active conformation that is characterized by the presence of two water molecules and a flip of the carbonyl of the conserved Asp251. The structure of the ternary complex of cyanide, L-arginine, and the oxygenase domain of inducible nitric oxide synthase was determined to 2.4A resolution. Cyanide binds essentially linearly, interacts with L-Arg, and induces the binding of a water molecule at the active site. This water is positioned by backbone interactions, located 2.8A from the nitrogen atom of cyanide, and could provide a proton required for O-O bond scission in the hydroxylation reaction of nitric oxide synthase.

About this StructureAbout this Structure

1N2N is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of cyanide complexes of P450cam and the oxygenase domain of inducible nitric oxide synthase-structural models of the short-lived oxygen complexes., Fedorov R, Ghosh DK, Schlichting I, Arch Biochem Biophys. 2003 Jan 1;409(1):25-31. PMID:12464241 Page seeded by OCA on Sat May 3 02:00:50 2008

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OCA

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 [[Image:1n2n.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1n2n", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=CYN:CYANIDE+ION'>CYN</scene>, <scene name='pdbligand=H4B:5,6,7,8-TETRAHYDROBIOPTERIN'>H4B</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitric-oxide_synthase Nitric-oxide synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.13.39 1.14.13.39] </span>
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-|GENE=
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-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n2n OCA], [http://www.ebi.ac.uk/pdbsum/1n2n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n2n RCSB]</span>
-}}
 '''Crystal structure of cyanide complex of the oxygenase domain of inducible nitric oxide synthase.'''
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 [[Category: Ghosh, D K.]]
 [[Category: Schlichting, I.]]
-[[Category: cyanide complex]]
+[[Category: Cyanide complex]]
-[[Category: nitric oxide synthase]]
+[[Category: Nitric oxide synthase]]
-[[Category: oxygen complex analogue]]
+[[Category: Oxygen complex analogue]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 02:00:50 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:24:32 2008''