1n06: Difference between revisions

Revision as of 01:55, 3 May 2008

Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold

OverviewOverview

The essential redox cofactors riboflavin monophosphate (FMN) and flavin adenine dinucleotide (FAD) are synthesised from their precursor, riboflavin, in sequential reactions by the metal-dependent riboflavin kinase and FAD synthetase. Here, we describe the 1.6A crystal structure of the Schizosaccharomyces pombe riboflavin kinase. The enzyme represents a novel family of phosphoryl transferring enzymes. It is a monomer comprising a central beta-barrel clasped on one side by two C-terminal helices that display an L-like shape. The opposite side of the beta-barrel serves as a platform for substrate binding as demonstrated by complexes with ADP and FMN. Formation of the ATP-binding site requires significant rearrangements in a short alpha-helix as compared to the substrate free form. The diphosphate moiety of ADP is covered by the glycine-rich flap I formed from parts of this alpha-helix. In contrast, no significant changes are observed upon binding of riboflavin. The ribityl side-chain might be covered by a rather flexible flap II. The unusual metal-binding site involves, in addition to the ADP phosphates, only the strictly conserved Thr45. This may explain the preference for zinc observed in vitro.

About this StructureAbout this Structure

1N06 is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of Schizosaccharomyces pombe riboflavin kinase reveals a novel ATP and riboflavin-binding fold., Bauer S, Kemter K, Bacher A, Huber R, Fischer M, Steinbacher S, J Mol Biol. 2003 Mar 7;326(5):1463-73. PMID:12595258 Page seeded by OCA on Sat May 3 01:55:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1n06.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1n06 |SIZE=350|CAPTION= <scene name='initialview01'>1n06</scene>, resolution 2.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1n06", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_kinase Riboflavin kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.26 2.7.1.26] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= SPCC18.16.C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])
+-->
-|DOMAIN=
+{{STRUCTURE_1n06|  PDB=1n06  |  SCENE=  }}
-|RELATEDENTRY=[[1n05|1N05]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1n06 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n06 OCA], [http://www.ebi.ac.uk/pdbsum/1n06 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1n06 RCSB]</span>
-}}
 '''Crystal Structure of Schizosaccharomyces pombe Riboflavin Kinase Reveals a Novel ATP and Riboflavin Binding Fold'''
@@ Line 32: / Line 29: @@
 [[Category: Kemter, K.]]
 [[Category: Steinbacher, S.]]
-[[Category: flavin cofactor]]
+[[Category: Flavin cofactor]]
-[[Category: kinase]]
+[[Category: Kinase]]
-[[Category: metal binding]]
+[[Category: Metal binding]]
-[[Category: phosphoryl transferase]]
+[[Category: Phosphoryl transferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:55:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:23:31 2008''