1mwt: Difference between revisions

Revision as of 01:49, 3 May 2008

Structure of penicillin G acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.45 A resolution.

OverviewOverview

The multiple antibiotic resistance of methicillin-resistant strains of Staphylococcus aureus (MRSA) has become a major clinical problem worldwide. The key determinant of the broad-spectrum beta-lactam resistance in MRSA strains is the penicillin-binding protein 2a (PBP2a). Because of its low affinity for beta-lactams, PBP2a provides transpeptidase activity to allow cell wall synthesis at beta-lactam concentrations that inhibit the beta-lactam-sensitive PBPs normally produced by S. aureus. The crystal structure of a soluble derivative of PBP2a has been determined to 1.8 A resolution and provides the highest resolution structure for a high molecular mass PBP. Additionally, structures of the acyl-PBP complexes of PBP2a with nitrocefin, penicillin G and methicillin allow, for the first time, a comparison of an apo and acylated resistant PBP. An analysis of the PBP2a active site in these forms reveals the structural basis of its resistance and identifies features in newly developed beta-lactams that are likely important for high affinity binding.

About this StructureAbout this Structure

1MWT is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus., Lim D, Strynadka NC, Nat Struct Biol. 2002 Nov;9(11):870-6. PMID:12389036 Page seeded by OCA on Sat May 3 01:48:58 2008

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OCA

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 [[Image:1mwt.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1mwt", creates the "Structure Box" on the page.
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-|RELATEDENTRY=[[1mwr|1MWR]], [[1mws|1MWS]], [[1mwu|1MWU]], [[1mwx|1MWX]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mwt OCA], [http://www.ebi.ac.uk/pdbsum/1mwt PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mwt RCSB]</span>
-}}
 '''Structure of penicillin G acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.45 A resolution.'''
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 [[Category: Lim, D C.]]
 [[Category: Strynadka, N C.J.]]
-[[Category: beta-lactam]]
+[[Category: Beta-lactam]]
-[[Category: d,d-carboxypeptidase]]
+[[Category: D,d-carboxypeptidase]]
-[[Category: d,d-transpeptidase]]
+[[Category: D,d-transpeptidase]]
-[[Category: penicillin binding protein]]
+[[Category: Penicillin binding protein]]
-[[Category: penicillin g]]
+[[Category: Penicillin g]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:48:58 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:22:16 2008''