1mvm: Difference between revisions

Revision as of 01:46, 3 May 2008

MVM(STRAIN I), COMPLEX(VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C

OverviewOverview

The three-dimensional crystal structure of the single-stranded DNA-containing ('full') parvovirus, minute virus of mice (MVM), has been determined to 3.5 A resolution. Both full and empty particles of MVM were crystallized in the monoclinic space group C2 with cell dimensions of a = 448.7, b = 416.7, c = 305.3 A and beta = 95.8 degrees. Diffraction data were collected at the Cornell High Energy Synchrotron Source using an oscillation camera. The crystals have a pseudo higher R32 space group in which the particles are situated at two special positions with 32 point symmetry, separated by (1/2)c in the hexagonal setting. The self-rotation function showed that the particles are rotated with respect to each other by 60 degrees around the pseudo threefold axis. Subsequently, a more detailed analysis of the structure amplitudes demonstrated that the correct space-group symmetry is C2 as given above. Only one of the three twofold axes perpendicular to the threefold axis in the pseudo R32 space group is a 'true' crystallographic twofold axis; the other two are only 'local' non-crystallographic symmetry axes. The known canine parvovirus (CPV) structure was used as a phasing model to initiate real-space electron-density averaging phase improvement. The electron density was easily interpretable and clearly showed the amino-acid differences between MVM and CPV, although the final overall correlation coefficient was only 0.63. The structure of MVM has a large amount of icosahedrally ordered DNA, amounting to 22% of the viral genome, which is significantly more than that found in CPV.

About this StructureAbout this Structure

1MVM is a Single protein structure of sequence from Minute virus of mice. Full crystallographic information is available from OCA.

ReferenceReference

Structure determination of minute virus of mice., Llamas-Saiz AL, Agbandje-McKenna M, Wikoff WR, Bratton J, Tattersall P, Rossmann MG, Acta Crystallogr D Biol Crystallogr. 1997 Jan 1;53(Pt 1):93-102. PMID:15299974 Page seeded by OCA on Sat May 3 01:46:30 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1mvm.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1mvm |SIZE=350|CAPTION= <scene name='initialview01'>1mvm</scene>, resolution 3.500&Aring;
+The line below this paragraph, containing "STRUCTURE_1mvm", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1mvm|  PDB=1mvm  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mvm OCA], [http://www.ebi.ac.uk/pdbsum/1mvm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mvm RCSB]</span>
-}}
 '''MVM(STRAIN I), COMPLEX(VIRAL COAT/DNA), VP2, PH=7.5, T=4 DEGREES C'''
@@ Line 27: / Line 24: @@
 [[Category: Agbandje-McKenna, M.]]
 [[Category: Llamas-Saiz, M G.Rossmann A L.]]
-[[Category: complex (viral coat protein/dna)]]
+[[Category: Icosahedral virus]]
-[[Category: icosahedral virus]]
+[[Category: Viral coat protein/nucleic acid]]
-[[Category: viral coat protein/nucleic acid]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:46:30 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:21:44 2008''