1mow: Difference between revisions

Revision as of 01:32, 3 May 2008

E-DreI

OverviewOverview

We have generated an artificial highly specific endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400 A(2) protein interface between these domains. Protein engineering was accomplished by combining computational redesign and an in vivo protein-folding screen. The resulting enzyme, E-DreI (Engineered I-DmoI/I-CreI), binds a long chimeric DNA target site with nanomolar affinity, cleaving it precisely at a rate equivalent to its natural parents. The structure of an E-DreI/DNA complex demonstrates the accuracy of the protein interface redesign algorithm and reveals how catalytic function is maintained during the creation of the new endonuclease. These results indicate that it may be possible to generate novel highly specific DNA binding proteins from homing endonucleases.

About this StructureAbout this Structure

1MOW is a Single protein structure of sequence from Desulfurococcus mobilis and chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

ReferenceReference

Design, activity, and structure of a highly specific artificial endonuclease., Chevalier BS, Kortemme T, Chadsey MS, Baker D, Monnat RJ, Stoddard BL, Mol Cell. 2002 Oct;10(4):895-905. PMID:12419232 Page seeded by OCA on Sat May 3 01:32:10 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1mow.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1mow |SIZE=350|CAPTION= <scene name='initialview01'>1mow</scene>, resolution 2.40&Aring;
+The line below this paragraph, containing "STRUCTURE_1mow", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1mow|  PDB=1mow  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mow OCA], [http://www.ebi.ac.uk/pdbsum/1mow PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mow RCSB]</span>
-}}
 '''E-DreI'''
@@ Line 31: / Line 28: @@
 [[Category: Kortemme, T.]]
 [[Category: Stoddard, B L.]]
-[[Category: design]]
+[[Category: Design]]
-[[Category: endonuclease]]
+[[Category: Endonuclease]]
-[[Category: engineering]]
+[[Category: Engineering]]
-[[Category: homing]]
+[[Category: Homing]]
-[[Category: laglidadg]]
+[[Category: Laglidadg]]
-[[Category: structure]]
+[[Category: Structure]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:32:10 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:19:01 2008''