1mjh: Difference between revisions

Revision as of 01:11, 3 May 2008

STRUCTURE-BASED ASSIGNMENT OF THE BIOCHEMICAL FUNCTION OF HYPOTHETICAL PROTEIN MJ0577: A TEST CASE OF STRUCTURAL GENOMICS

OverviewOverview

Many small bacterial, archaebacterial, and eukaryotic genomes have been sequenced, and the larger eukaryotic genomes are predicted to be completely sequenced within the next decade. In all genomes sequenced to date, a large portion of these organisms' predicted protein coding regions encode polypeptides of unknown biochemical, biophysical, and/or cellular functions. Three-dimensional structures of these proteins may suggest biochemical or biophysical functions. Here we report the crystal structure of one such protein, MJ0577, from a hyperthermophile, Methanococcus jannaschii, at 1.7-A resolution. The structure contains a bound ATP, suggesting MJ0577 is an ATPase or an ATP-mediated molecular switch, which we confirm by biochemical experiments. Furthermore, the structure reveals different ATP binding motifs that are shared among many homologous hypothetical proteins in this family. This result indicates that structure-based assignment of molecular function is a viable approach for the large-scale biochemical assignment of proteins and for discovering new motifs, a basic premise of structural genomics.

About this StructureAbout this Structure

1MJH is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

ReferenceReference

Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics., Zarembinski TI, Hung LW, Mueller-Dieckmann HJ, Kim KK, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15189-93. PMID:9860944 Page seeded by OCA on Sat May 3 01:11:31 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1mjh.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1mjh |SIZE=350|CAPTION= <scene name='initialview01'>1mjh</scene>, resolution 1.7&Aring;
+The line below this paragraph, containing "STRUCTURE_1mjh", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= MJ0577 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00293 USP_Like]</span>
+{{STRUCTURE_1mjh|  PDB=1mjh  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjh OCA], [http://www.ebi.ac.uk/pdbsum/1mjh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mjh RCSB]</span>
-}}
 '''STRUCTURE-BASED ASSIGNMENT OF THE BIOCHEMICAL FUNCTION OF HYPOTHETICAL PROTEIN MJ0577: A TEST CASE OF STRUCTURAL GENOMICS'''
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 [[Category: Yokota, H.]]
 [[Category: Zarembinski, T I.]]
-[[Category: atp binding protein]]
+[[Category: Atp binding protein]]
-[[Category: berkeley structural genomics center]]
+[[Category: Berkeley structural genomics center]]
-[[Category: bsgc structure funded by nih]]
+[[Category: Bsgc structure funded by nih]]
-[[Category: functional assignment]]
+[[Category: Functional assignment]]
-[[Category: hypothetical protein]]
+[[Category: Hypothetical protein]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:11:31 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:52 2008''