1mj1: Difference between revisions

Revision as of 01:08, 3 May 2008

FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME

OverviewOverview

The mRNA codon in the ribosomal A-site is recognized by aminoacyl-tRNA (aa-tRNA) in a ternary complex with elongation factor Tu (EF-Tu) and GTP. Here we report the 13 A resolution three-dimensional reconstruction determined by cryo-electron microscopy of the kirromycin-stalled codon-recognition complex. The structure of the ternary complex is distorted by binding of the tRNA anticodon arm in the decoding center. The aa-tRNA interacts with 16S rRNA, helix 69 of 23S rRNA and proteins S12 and L11, while the sarcin-ricin loop of 23S rRNA contacts domain 1 of EF-Tu near the nucleotide-binding pocket. These results provide a detailed snapshot view of an important functional state of the ribosome and suggest mechanisms of decoding and GTPase activation.

About this StructureAbout this Structure

1MJ1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex., Stark H, Rodnina MV, Wieden HJ, Zemlin F, Wintermeyer W, van Heel M, Nat Struct Biol. 2002 Nov;9(11):849-54. PMID:12379845 Page seeded by OCA on Sat May 3 01:08:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1mj1.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1mj1", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1mj1|  PDB=1mj1  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mj1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mj1 OCA], [http://www.ebi.ac.uk/pdbsum/1mj1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mj1 RCSB]</span>
-}}
 '''FITTING THE TERNARY COMPLEX OF EF-Tu/tRNA/GTP AND RIBOSOMAL PROTEINS INTO A 13 A CRYO-EM MAP OF THE COLI 70S RIBOSOME'''
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 [[Category: Zemlin, F.]]
 [[Category: 70s ribosome]]
-[[Category: ef-tu]]
+[[Category: Ef-tu]]
-[[Category: low resolution model ternary complex]]
+[[Category: Low resolution model ternary complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:08:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:37 2008''