1miw: Difference between revisions

Revision as of 01:08, 3 May 2008

Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with ATP

OverviewOverview

CCA-adding enzymes polymerize CCA onto the 3' terminus of immature tRNAs without using a nucleic acid template. The 3.0 A resolution crystal structures of the CCA-adding enzyme from Bacillus stearothermophilus and its complexes with ATP or CTP reveal a seahorse-shaped subunit consisting of four domains: head, neck, body, and tail. The head is structurally homologous to the palm domain of DNA polymerase beta but has additional structural features and functions. The neck, body, and tail represent new protein folding motifs. The neck provides a specific template for the incoming ATP or CTP, whereas the body and tail may bind tRNA. Each subunit has one active site capable of switching its base specificity between ATP and CTP, an important component of the CCA-adding mechanism.

About this StructureAbout this Structure

1MIW is a Single protein structure of sequence from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of the Bacillus stearothermophilus CCA-adding enzyme and its complexes with ATP or CTP., Li F, Xiong Y, Wang J, Cho HD, Tomita K, Weiner AM, Steitz TA, Cell. 2002 Dec 13;111(6):815-24. PMID:12526808 Page seeded by OCA on Sat May 3 01:08:15 2008

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OCA

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 [[Image:1miw.gif|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1miw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1miw OCA], [http://www.ebi.ac.uk/pdbsum/1miw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1miw RCSB]</span>
-}}
 '''Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with ATP'''
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 [[Category: Weiner, A M.]]
 [[Category: Xiong, Y.]]
-[[Category: cca-adding enzyme]]
+[[Category: Cca-adding enzyme]]
-[[Category: trna nucleotidyltransferase]]
+[[Category: Trna nucleotidyltransferase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 01:08:15 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:16:35 2008''