1mfa: Difference between revisions

Revision as of 00:57, 3 May 2008

STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION

OverviewOverview

We describe here the 1.7-A resolution structure of a single-chain antibody variable domain (scFv) molecule, based on the carbohydrate-binding antibody Se155-4, complexed with the trisaccharide ligand alpha-D-Gal(1-->2)[alpha-D-Abe(1-->3)]alpha-D-Manp1-->OMe, where Abe is abequose. The scFv expressed in Escherichia coli has the variable region light chain to heavy chain polarity with the domains connected by a 19-residue linker. Although the linker is partially disordered in the crystal, the packing of the molecules suggests a monomeric state of the scFv. The carbohydrate adopts a different conformation about the Man-Gal linkage than was observed previously in the Fab-trisaccharide complex. Instead of a direct hydrogen bond between O2Abe and O2Gal, these two atoms are bridged by a water molecule in the present complex.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Structure of a single-chain antibody variable domain (Fv) fragment complexed with a carbohydrate antigen at 1.7-A resolution., Zdanov A, Li Y, Bundle DR, Deng SJ, MacKenzie CR, Narang SA, Young NM, Cygler M, Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6423-7. PMID:7517550 Page seeded by OCA on Sat May 3 00:57:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1mfa.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1mfa", creates the "Structure Box" on the page.
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+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ABE:ABEQUOSE'>ABE</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>
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-|GENE=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mfa OCA], [http://www.ebi.ac.uk/pdbsum/1mfa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mfa RCSB]</span>
-}}
 '''STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION'''
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 ==About this Structure==
-MFA is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFA OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MFA OCA].
 ==Reference==
 Structure of a single-chain antibody variable domain (Fv) fragment complexed with a carbohydrate antigen at 1.7-A resolution., Zdanov A, Li Y, Bundle DR, Deng SJ, MacKenzie CR, Narang SA, Young NM, Cygler M, Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6423-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7517550 7517550]
-[[Category: Mus musculus]]
-[[Category: Protein complex]]
 [[Category: Cygler, M.]]
 [[Category: Zdanov, A.]]
-[[Category: immunoglobulin]]
+[[Category: Immunoglobulin]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:57:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:15:24 2008''