1mee: Difference between revisions

Revision as of 00:56, 3 May 2008

THE COMPLEX BETWEEN THE SUBTILISIN FROM A MESOPHILIC BACTERIUM AND THE LEECH INHIBITOR EGLIN-C

OverviewOverview

The alkaline proteinase from the mesophilic bacterium Bacillus mesentericus has been crystallized in a 1:1 complex with the inhibitor eglin-C from the medical leech. The crystals have cell dimensions of a = 43.0, b = 71.9, c = 48.3 A and beta = 110.0 degrees and are in the space group P2(1). Three-dimensional data to 2.0 A have been recorded on film from a single crystal. The orientation and position of the complex in the unit cell have been established using the refined coordinates of subtilisin Carlsberg and of eglin-C as independent models. The structure of the complex has been refined by restrained least-squares minimization. The crystallographic R factor (= sigma[magnitude of Fo - magnitude of Fc[/sigma magnitude of Fo) is 15.1% including two Ca2+ ions and 312 water molecules. The structure is discussed in terms of its physicochemical properties in solution and its relation to other Bacillus subtilisins.

About this StructureAbout this Structure

1MEE is a Protein complex structure of sequences from Bacillus pumilus and Hirudo medicinalis. Full crystallographic information is available from OCA.

ReferenceReference

Complex between the subtilisin from a mesophilic bacterium and the leech inhibitor eglin-C., Dauter Z, Betzel C, Genov N, Pipon N, Wilson KS, Acta Crystallogr B. 1991 Oct 1;47 ( Pt 5):707-30. PMID:1793542 Page seeded by OCA on Sat May 3 00:56:25 2008

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OCA

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 [[Image:1mee.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1mee", creates the "Structure Box" on the page.
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span>
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-}}
 '''THE COMPLEX BETWEEN THE SUBTILISIN FROM A MESOPHILIC BACTERIUM AND THE LEECH INHIBITOR EGLIN-C'''
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 [[Category: Dauter, Z.]]
 [[Category: Wilson, K S.]]
-[[Category: complex(serine proteinase-inhibitor)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:56:25 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:15:02 2008''