1mcv: Difference between revisions

Revision as of 00:53, 3 May 2008

Crystal Structure Analysis of a Hybrid Squash Inhibitor in Complex with Porcine Pancreatic Elastase

OverviewOverview

The crystal structure of porcine pancreatic elastase in complex with a hybrid squash inhibitor (HEI-TOE I; 28 amino acids) has been determined to a resolution of 1.8 A. To construct the hybrid inhibitor, the trypsin-binding loop of the squash inhibitor from Ecballium elaterium was substituted by the sequence of a peptide that was derived from the third domain of the turkey ovomucoid inhibitor and was optimized to inhibit porcine pancreatic elastase. This modification of the squash inhibitor changed its specificity for trypsin to a specificity for porcine pancreatic elastase. Specific interactions of this hybrid inhibitor with porcine pancreatic elastase and the differences from the interactions of the ovomucoid inhibitor with human leukocyte elastase are discussed. The binding loop of the inhibitor adopts a 'canonical' conformation and the scissile bond Leu-Glu remains intact.

About this StructureAbout this Structure

1MCV is a Single protein structure of sequence from Sus scrofa. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a hybrid squash inhibitor in complex with porcine pancreatic elastase at 1.8 A resolution., Ay J, Hilpert K, Krauss N, Schneider-Mergener J, Hohne W, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):247-54. Epub 2003, Jan 23. PMID:12554935 Page seeded by OCA on Sat May 3 00:53:46 2008

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OCA

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 [[Image:1mcv.jpg|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mcv OCA], [http://www.ebi.ac.uk/pdbsum/1mcv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mcv RCSB]</span>
-}}
 '''Crystal Structure Analysis of a Hybrid Squash Inhibitor in Complex with Porcine Pancreatic Elastase'''
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 [[Category: Krauss, N.]]
 [[Category: Schneider-Mergener, J.]]
-[[Category: elastase-inhibitor complex]]
+[[Category: Elastase-inhibitor complex]]
-[[Category: hybrid squash inhibitor]]
+[[Category: Hybrid squash inhibitor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:53:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:14:33 2008''