3syn: Difference between revisions
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==Crystal structure of FlhF in complex with its activator== | ==Crystal structure of FlhF in complex with its activator== | ||
<StructureSection load='3syn' size='340' side='right' caption='[[3syn]], [[Resolution|resolution]] 3.06Å' scene=''> | <StructureSection load='3syn' size='340' side='right'caption='[[3syn]], [[Resolution|resolution]] 3.06Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3syn]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SYN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SYN FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2px0|2px0]], [[2px3|2px3]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2px0|2px0]], [[2px3|2px3]]</div></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flhF, BSU16400 ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flhF, BSU16400 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3syn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3syn OCA], [https://pdbe.org/3syn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3syn RCSB], [https://www.ebi.ac.uk/pdbsum/3syn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3syn ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[ | [[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU]] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum. [[https://www.uniprot.org/uniprot/YLXH_BACSU YLXH_BACSU]] Involved in the placement and assembly of flagella (By similarity). Activates the SRP-GTPase activity of FlhF.<ref>PMID:22056770</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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==See Also== | ==See Also== | ||
*[[GTP-binding protein|GTP-binding protein]] | *[[Flagellar biosynthetic protein|Flagellar biosynthetic protein]] | ||
*[[Flagellar protein 3D structures|Flagellar protein 3D structures]] | |||
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Vibrio subtilis ehrenberg 1835]] | ||
[[Category: Large Structures]] | |||
[[Category: Bange, G]] | [[Category: Bange, G]] | ||
[[Category: Kuemmerer, N]] | [[Category: Kuemmerer, N]] | ||
Revision as of 11:16, 29 June 2022
Crystal structure of FlhF in complex with its activatorCrystal structure of FlhF in complex with its activator
Structural highlights
Function[FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum. [YLXH_BACSU] Involved in the placement and assembly of flagella (By similarity). Activates the SRP-GTPase activity of FlhF.[1] Publication Abstract from PubMedSmall G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here we show that the YlxH protein activates the SRP-GTPase FlhF. The crystal structure of the Bacillus subtilis FlhF-effector complex revealed that the effector does not contribute a catalytic residue but positions the catalytic machinery already present in SRP-GTPases. We provide a general concept that might also apply to the RNA-driven activation of the universally conserved, co-translational protein-targeting machinery comprising the SRP-GTPases Ffh and FtsY. Our study exemplifies the evolutionary transition from RNA- to protein-driven activation in SRP-GTPases and suggests that the current view on SRP-mediated protein targeting is incomplete. Structural basis for the molecular evolution of SRP-GTPase activation by protein.,Bange G, Kummerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I Nat Struct Mol Biol. 2011 Nov 6;18(12):1376-80. doi: 10.1038/nsmb.2141. PMID:22056770[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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