3zgi: Difference between revisions
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==Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP== | ==Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP== | ||
<StructureSection load='3zgi' size='340' side='right' caption='[[3zgi]], [[Resolution|resolution]] 2.25Å' scene=''> | <StructureSection load='3zgi' size='340' side='right'caption='[[3zgi]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3zgi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZGI FirstGlance]. <br> | <table><tr><td colspan='2'>[[3zgi]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Strpn Strpn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZGI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZGI FirstGlance]. <br> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Strpn]] | [[Category: Strpn]] | ||
[[Category: Achour, A]] | [[Category: Achour, A]] | ||
Revision as of 15:30, 10 May 2019
Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrPCrystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP
Structural highlights
Publication Abstract from PubMedStreptococcus pneumoniae is a major human pathogen, and a leading cause of disease and death worldwide. Pneumococcal invasive disease is triggered by initial asymptomatic colonization of the human upper respiratory tract. The pneumococcal serine-rich repeat protein (PsrP) is a lung-specific virulence factor whose functional binding region (BR) binds to keratin-10 (KRT10) and promotes pneumococcal biofilm formation through self-oligomerization. We present the crystal structure of the KRT10-binding domain of PsrP (BR187-385) determined to 2.0 A resolution. BR187-385 adopts a novel variant of the DEv-IgG fold, typical for microbial surface components recognizing adhesive matrix molecules adhesins, despite very low sequence identity. An extended beta-sheet on one side of the compressed, two-sided barrel presents a basic groove that possibly binds to the acidic helical rod domain of KRT10. Our study also demonstrates the importance of the other side of the barrel, formed by extensive well-ordered loops and stabilized by short beta-strands, for interaction with KRT10. The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold.,Schulte T, Lofling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A Open Biol. 2014 Jan 15;4(1):130090. doi: 10.1098/rsob.130090. PMID:24430336[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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