1m3q: Difference between revisions

Revision as of 00:35, 3 May 2008

Crystal Structure of hogg1 D268E Mutant with Base-Excised DNA and 8-aminoguanine

OverviewOverview

DNA glycosylase/lyases initiate the repair of damaged nucleobases in the genome by catalyzing excision of aberrant nucleobases and nicking of the lesion-containing DNA strand. Nearly all of these proteins have the unusual property of remaining tightly bound in vitro to the end products of the reaction cascade. We have taken advantage of this property to crystallize and structurally characterize the end product resulting from complete DNA processing by a catalytically active mutant form of human 8-oxoguanine DNA glycosylase (D268E hOgg1). The resulting structure is consistent with the currently accepted catalytic mechanism for the protein. Unexpectedly, however, soaking of a nucleobase analog into the crystals results in religation of the DNA backbone in situ.

About this StructureAbout this Structure

1M3Q is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structures of end products resulting from lesion processing by a DNA glycosylase/lyase., Chung SJ, Verdine GL, Chem Biol. 2004 Dec;11(12):1643-9. PMID:15610848 Page seeded by OCA on Sat May 3 00:35:29 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1m3q.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1m3q |SIZE=350|CAPTION= <scene name='initialview01'>1m3q</scene>, resolution 1.90&Aring;
+The line below this paragraph, containing "STRUCTURE_1m3q", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ANG:8-AMINOGUANINE'>ANG</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DRZ:3&#39;,4&#39;-DIHYDROXY-PENTANAL-5&#39;-PHOSPHATE'>DRZ</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= ogg1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_1m3q|  PDB=1m3q  |  SCENE=  }}
-|RELATEDENTRY=[[1ebm|1EBM]], [[1m3h|1M3H]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1m3q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1m3q OCA], [http://www.ebi.ac.uk/pdbsum/1m3q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1m3q RCSB]</span>
-}}
 '''Crystal Structure of hogg1 D268E Mutant with Base-Excised DNA and 8-aminoguanine'''
@@ Line 29: / Line 26: @@
 [[Category: 8-aminoguanine]]
 [[Category: 8-oxoguanine]]
-[[Category: dna glycosylase]]
+[[Category: Dna glycosylase]]
-[[Category: dna repair]]
+[[Category: Dna repair]]
-[[Category: end product]]
+[[Category: End product]]
-[[Category: hogg]]
+[[Category: Hogg]]
-[[Category: re-ligation]]
+[[Category: Re-ligation]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:35:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:10:49 2008''