1m15: Difference between revisions

Revision as of 00:30, 3 May 2008

TRANSITION STATE STRUCTURE OF ARGININE KINASE

OverviewOverview

The three-dimensional crystal structure of an arginine kinase transition-state analogue complex has been refined at 1.2 A resolution, with an overall R factor of 12.3%. The current model provides a unique opportunity to analyze the structure of a bimolecular (phosphagen kinase) enzyme in its transition state. This atomic resolution structure confirms in-line transfer of the phosphoryl group and the catalytic importance of the precise alignment of the substrates. The structure is consistent with a concerted proton transfer that has been proposed for an unrelated kinase. Refinement of anisotropic temperature factors and translation-libration-screw (TLS) analyses led to the identification of four rigid groups and their prevalent modes of motion in the transition state. The relative magnitudes of the mobility of rigid groups are consistent with their proposed roles in catalysis.

About this StructureAbout this Structure

1M15 is a Single protein structure of sequence from Limulus polyphemus. Full crystallographic information is available from OCA.

ReferenceReference

Refinement of the arginine kinase transition-state analogue complex at 1.2 A resolution: mechanistic insights., Yousef MS, Fabiola F, Gattis JL, Somasundaram T, Chapman MS, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2009-17. Epub 2002, Nov 23. PMID:12454458 Page seeded by OCA on Sat May 3 00:30:19 2008

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OCA

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 [[Image:1m15.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1m15", creates the "Structure Box" on the page.
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-}}
 '''TRANSITION STATE STRUCTURE OF ARGININE KINASE'''
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 [[Category: Somasundaram, T.]]
 [[Category: Yousef, M S.]]
-[[Category: adenosine triphosphate]]
+[[Category: Adenosine triphosphate]]
-[[Category: arginine kinase]]
+[[Category: Arginine kinase]]
-[[Category: creatine kinase]]
+[[Category: Creatine kinase]]
-[[Category: phosphagen kinase]]
+[[Category: Phosphagen kinase]]
-[[Category: transition state analog]]
+[[Category: Transition state analog]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:30:19 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:09:55 2008''