4bw0: Difference between revisions
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==The molecular recognition of kink turn structure by the L7Ae class of proteins== | ==The molecular recognition of kink turn structure by the L7Ae class of proteins== | ||
<StructureSection load='4bw0' size='340' side='right' caption='[[4bw0]], [[Resolution|resolution]] 2.33Å' scene=''> | <StructureSection load='4bw0' size='340' side='right'caption='[[4bw0]], [[Resolution|resolution]] 2.33Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4bw0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BW0 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4bw0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arcfl Arcfl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4BW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4BW0 FirstGlance]. <br> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Arcfl]] | [[Category: Arcfl]] | ||
[[Category: Large Structures]] | |||
[[Category: Huang, L]] | [[Category: Huang, L]] | ||
[[Category: Lilley, D M.J]] | [[Category: Lilley, D M.J]] | ||
[[Category: Rna-rna binding protein complex]] | [[Category: Rna-rna binding protein complex]] | ||
Revision as of 16:10, 13 March 2019
The molecular recognition of kink turn structure by the L7Ae class of proteinsThe molecular recognition of kink turn structure by the L7Ae class of proteins
Structural highlights
Function[RL7A_ARCFU] Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA and box C/D sRNAs.[1] Publication Abstract from PubMedL7Ae is a member of a protein family that binds kink-turns (k-turns) in many functional RNA species. We have solved the X-ray crystal structure of the near-consensus sequence Kt-7 of Haloarcula marismortui bound by Archaeoglobus fulgidus L7Ae at 2.3-A resolution. We also present a structure of Kt-7 in the absence of bound protein at 2.2-A resolution. As a result, we can describe a general mode of recognition of k-turn structure by the L7Ae family proteins. The protein makes interactions in the widened major groove on the outer face of the k-turn. Two regions of the protein are involved. One is an alpha-helix that enters the major groove of the NC helix, making both nonspecific backbone interactions and specific interactions with the guanine nucleobases of the conserved G*A pairs. A hydrophobic loop makes close contact with the L1 and L2 bases, and a glutamate side chain hydrogen bonds with L1. Taken together, these interactions are highly selective for the structure of the k-turn and suggest how conformational selection of the folded k-turn occurs. The molecular recognition of kink-turn structure by the L7Ae class of proteins.,Huang L, Lilley DM RNA. 2013 Oct 22. PMID:24149842[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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