1lrr: Difference between revisions

Revision as of 00:13, 3 May 2008

CRYSTAL STRUCTURE OF E. COLI SEQA COMPLEXED WITH HEMIMETHYLATED DNA

OverviewOverview

The SeqA protein binds clusters of fully methylated or hemimethylated GATC sequences at oriC and negatively modulates the initiation of DNA replication. We find that SeqA can be proteolytically cleaved into an N-terminal multimerization and a C-terminal DNA-binding domain and have determined the crystal structure of the C-terminal domain in complex with a hemimethylated GATC site. SeqA makes direct hydrogen bonds and van der Waals contacts with the hemimethylated A-T base pair in addition to interactions with the surrounding bases and DNA backbone. The tetrameric protein-DNA complex found in the crystal suggests that SeqA binds multiple GATC sites on separate DNA duplexes, altering the overall DNA topology and sequestering oriC from replication initiation.

About this StructureAbout this Structure

1LRR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Insights into negative modulation of E. coli replication initiation from the structure of SeqA-hemimethylated DNA complex., Guarne A, Zhao Q, Ghirlando R, Yang W, Nat Struct Biol. 2002 Nov;9(11):839-43. PMID:12379844 Page seeded by OCA on Sat May 3 00:13:19 2008

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OCA

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 [[Image:1lrr.gif|left|200px]]
- {{Structure
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-|GENE= seqA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lrr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lrr OCA], [http://www.ebi.ac.uk/pdbsum/1lrr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lrr RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF E. COLI SEQA COMPLEXED WITH HEMIMETHYLATED DNA'''
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 [[Category: Yang, W.]]
 [[Category: Zhao, Q.]]
-[[Category: methylated gatc]]
+[[Category: Methylated gatc]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
-[[Category: replication]]
+[[Category: Replication]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:13:19 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:06:31 2008''