1lp1: Difference between revisions

Revision as of 00:08, 3 May 2008

Protein Z in complex with an in vitro selected affibody

OverviewOverview

The broad binding repertoire of antibodies has permitted their use in a wide range of applications. However, some uses of antibodies are precluded due to limitations in the efficiency of antibody generation. In vitro evolved binding proteins, selected from combinatorial libraries generated around various alternative structural scaffolds, are promising alternatives to antibodies. We have solved the crystal structure of a complex of an all alpha-helical in vitro selected binding protein (affibody) bound to protein Z, an IgG Fc-binding domain derived from staphylococcal protein A. The structure of the complex reveals an extended and complementary binding surface with similar properties to protein-antibody interactions. The surface region of protein Z recognized by the affibody is strikingly similar to the one used for IgG(1) Fc binding, suggesting that this surface contains potential hot-spots for binding. The implications of the selected affibody binding-mode for its application as a universal binding protein are discussed.

About this StructureAbout this Structure

1LP1 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for recognition by an in vitro evolved affibody., Hogbom M, Eklund M, Nygren PA, Nordlund P, Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3191-6. Epub 2003 Feb 25. PMID:12604795 Page seeded by OCA on Sat May 3 00:08:11 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1lp1.jpg|left|200px]]
- {{Structure
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lp1 OCA], [http://www.ebi.ac.uk/pdbsum/1lp1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lp1 RCSB]</span>
-}}
 '''Protein Z in complex with an in vitro selected affibody'''
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 [[Category: Nordlund, P.]]
 [[Category: Nygren, P A.]]
-[[Category: affibody]]
+[[Category: Affibody]]
-[[Category: in vitro evolved]]
+[[Category: In vitro evolved]]
-[[Category: protein-protein complex]]
+[[Category: Protein-protein complex]]
-[[Category: three-helix bundle]]
+[[Category: Three-helix bundle]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:08:11 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:05:34 2008''