1los: Difference between revisions

Revision as of 00:07, 3 May 2008

crystal structure of orotidine monophosphate decarboxylase mutant deltaR203A complexed with 6-azaUMP

OverviewOverview

The crystal structures of the enzyme orotidine-5'-monophosphate decarboxylase from Methanobacterium thermoautotrophicum complexed with its product UMP and the inhibitors 6-hydroxyuridine 5'-phosphate (BMP), XMP, and CMP are reported. A mutant version of the protein, in which four residues of the flexible phosphate-binding loop (180)Gly-Gly(190) were removed and Arg(203) was replaced by alanine, was also analyzed. The XMP and CMP complexes reveal a ligand-binding mode that is distinct from the one identified previously with the aromatic rings located outside the binding pocket. A potential pathway for ligand binding is discussed.

About this StructureAbout this Structure

1LOS is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of inhibitor complexes reveal an alternate binding mode in orotidine-5'-monophosphate decarboxylase., Wu N, Pai EF, J Biol Chem. 2002 Aug 2;277(31):28080-7. Epub 2002 May 13. PMID:12011084 Page seeded by OCA on Sat May 3 00:07:46 2008

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OCA

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 [[Image:1los.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1los", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=UP6:6-AZA+URIDINE+5&#39;-MONOPHOSPHATE'>UP6</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Orotidine-5'-phosphate_decarboxylase Orotidine-5'-phosphate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.23 4.1.1.23] </span>
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-|RELATEDENTRY=[[1lol|1LOL]], [[1loq|1LOQ]], [[1lor|1LOR]], [[1lp6|1LP6]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1los FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1los OCA], [http://www.ebi.ac.uk/pdbsum/1los PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1los RCSB]</span>
-}}
 '''crystal structure of orotidine monophosphate decarboxylase mutant deltaR203A complexed with 6-azaUMP'''
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 [[Category: Pai, E F.]]
 [[Category: Wu, N.]]
-[[Category: tim barrel]]
+[[Category: Tim barrel]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:07:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:05:26 2008''