1lnq: Difference between revisions

Revision as of 00:05, 3 May 2008

CRYSTAL STRUCTURE OF MTHK AT 3.3 A

OverviewOverview

Ion channels exhibit two essential biophysical properties; that is, selective ion conduction, and the ability to gate-open in response to an appropriate stimulus. Two general categories of ion channel gating are defined by the initiating stimulus: ligand binding (neurotransmitter- or second-messenger-gated channels) or membrane voltage (voltage-gated channels). Here we present the structural basis of ligand gating in a K(+) channel that opens in response to intracellular Ca(2+). We have cloned, expressed, analysed electrical properties, and determined the crystal structure of a K(+) channel (MthK) from Methanobacterium thermoautotrophicum in the Ca(2+)-bound, opened state. Eight RCK domains (regulators of K(+) conductance) form a gating ring at the intracellular membrane surface. The gating ring uses the free energy of Ca(2+) binding in a simple manner to perform mechanical work to open the pore.

About this StructureAbout this Structure

1LNQ is a Single protein structure of sequence from Methanothermobacter thermautotrophicus. The following page contains interesting information on the relation of 1LNQ with [Potassium Channels]. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure and mechanism of a calcium-gated potassium channel., Jiang Y, Lee A, Chen J, Cadene M, Chait BT, MacKinnon R, Nature. 2002 May 30;417(6888):515-22. PMID:12037559 Page seeded by OCA on Sat May 3 00:05:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1lnq.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1lnq |SIZE=350|CAPTION= <scene name='initialview01'>1lnq</scene>, resolution 3.30&Aring;
+The line below this paragraph, containing "STRUCTURE_1lnq", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= mth1520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 Methanothermobacter thermautotrophicus])
+-->
-|DOMAIN=
+{{STRUCTURE_1lnq|  PDB=1lnq  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lnq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lnq OCA], [http://www.ebi.ac.uk/pdbsum/1lnq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lnq RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF MTHK AT 3.3 A'''
@@ Line 32: / Line 29: @@
 [[Category: Lee, A.]]
 [[Category: Mackinnon, R.]]
-[[Category: helix bundle]]
+[[Category: Helix bundle]]
-[[Category: membrane protein]]
+[[Category: Membrane protein]]
-[[Category: rossman fold]]
+[[Category: Rossman fold]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:05:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:05:01 2008''