1lmv: Difference between revisions

Revision as of 00:04, 3 May 2008

Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae

OverviewOverview

Pairing of a consensus sequence of the precursor (pre)-mRNA intron with a short region of the U2 small nuclear (sn)RNA during assembly of the eukaryotic spliceosome results in formation of a complementary helix of seven base pairs with a single unpaired adenosine residue. The 2' OH of this adenosine, called the branch site, brings about nucleophilic attack at the pre-mRNA 5' splice site in the first step of splicing. Another feature of this pairing is the phylogenetic conservation of a pseudouridine (psi) residue in U2 snRNA nearly opposite the branch site. We show that the presence of this psi in the pre-mRNA branch-site helix of Saccharomyces cerevisiae induces a dramatically altered architectural landscape compared with that of its unmodified counterpart. The psi-induced structure places the nucleophile in an accessible position for the first step of splicing.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine., Newby MI, Greenbaum NL, Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:12426583 Page seeded by OCA on Sat May 3 00:04:32 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1lmv.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1lmv |SIZE=350|CAPTION= <scene name='initialview01'>1lmv</scene>
+The line below this paragraph, containing "STRUCTURE_1lmv", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1lmv|  PDB=1lmv  |  SCENE=  }}
-|RELATEDENTRY=[[1lpw|1LPW]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lmv OCA], [http://www.ebi.ac.uk/pdbsum/1lmv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1lmv RCSB]</span>
-}}
 '''Solution structure of the unmodified U2 snRNA-intron branch site helix from S. cerevisiae'''
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 ==About this Structure==
-LMV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMV OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMV OCA].
 ==Reference==
 Sculpting of the spliceosomal branch site recognition motif by a conserved pseudouridine., Newby MI, Greenbaum NL, Nat Struct Biol. 2002 Dec;9(12):958-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12426583 12426583]
-[[Category: Protein complex]]
-[[Category: Saccharomyces cerevisiae]]
 [[Category: Greenbaum, N L.]]
 [[Category: Newby, M I.]]
-[[Category: a-form helix]]
+[[Category: A-form helix]]
-[[Category: branch site]]
+[[Category: Branch site]]
-[[Category: nmr]]
+[[Category: Nmr]]
-[[Category: solution structure]]
+[[Category: Solution structure]]
-[[Category: u2 snrna]]
+[[Category: U2 snrna]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 00:04:32 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:04:46 2008''