1kyu: Difference between revisions

Revision as of 23:20, 2 May 2008

AP-2 CLATHRIN ADAPTOR ALPHA-APPENDAGE IN COMPLEX WITH EPS15 DPF PEPTIDE

OverviewOverview

Clathrin-mediated endocytosis depends upon the interaction of accessory proteins with the alpha-ear of the AP-2 adaptor. We present structural characterization of these regulatory interactions. DPF and DPW motif peptides derived from eps15 and epsin bind in type I beta turn conformations to a conserved pocket on the alpha-ear platform. We show evidence for a second binding site that is DPW motif specific. The structure of a complex with an AP-2 binding segment from amphiphysin reveals a novel binding motif that we term FxDxF, which is engaged in an extended conformation by a unique surface of the platform domain. The FxDxF motif is also used by AP180 and the 170 kDa isoform of synaptojanin and can be found in several potential endocytic proteins, including HIP1, CD2AP, and PLAP. A mechanism of clathrin assembly regulation is suggested by three different AP-2 engagement modes.

About this StructureAbout this Structure

1KYU is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

Accessory protein recruitment motifs in clathrin-mediated endocytosis., Brett TJ, Traub LM, Fremont DH, Structure. 2002 Jun;10(6):797-809. PMID:12057195 Page seeded by OCA on Fri May 2 23:20:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1kyu.jpg|left|200px]]
- {{Structure
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-|RELATEDENTRY=[[1qtp|1QTP]], [[1qts|1QTS]], [[1ky6|1KY6]], [[1ky7|1KY7]], [[1kyd|1KYD]], [[1kyf|1KYF]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kyu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kyu OCA], [http://www.ebi.ac.uk/pdbsum/1kyu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kyu RCSB]</span>
-}}
 '''AP-2 CLATHRIN ADAPTOR ALPHA-APPENDAGE IN COMPLEX WITH EPS15 DPF PEPTIDE'''
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 [[Category: Fremont, D H.]]
 [[Category: Traub, L M.]]
-[[Category: endocytosis]]
+[[Category: Endocytosis]]
-[[Category: protein-peptide complex]]
+[[Category: Protein-peptide complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:20:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:55:34 2008''