1kq4: Difference between revisions

Revision as of 23:02, 2 May 2008

Crystal Structure of Thy1-Complementing Protein (TM0449) from Thermotoga maritima at 2.25 A Resolution

OverviewOverview

Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to the proteome of the thermophilic bacterium Thermotoga maritima. By using this pipeline, we successfully cloned and attempted expression of 1,376 of the predicted 1,877 genes (73%) and have identified crystallization conditions for 432 proteins, comprising 23% of the T. maritima proteome. Representative structures from TM0423 glycerol dehydrogenase and TM0449 thymidylate synthase-complementing protein are presented as examples of final outputs from the pipeline.

About this StructureAbout this Structure

1KQ4 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.

ReferenceReference

Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline., Lesley SA, Kuhn P, Godzik A, Deacon AM, Mathews I, Kreusch A, Spraggon G, Klock HE, McMullan D, Shin T, Vincent J, Robb A, Brinen LS, Miller MD, McPhillips TM, Miller MA, Scheibe D, Canaves JM, Guda C, Jaroszewski L, Selby TL, Elsliger MA, Wooley J, Taylor SS, Hodgson KO, Wilson IA, Schultz PG, Stevens RC, Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11664-9. Epub 2002 Aug 22. PMID:12193646 Page seeded by OCA on Fri May 2 23:02:07 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1kq4.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1kq4 |SIZE=350|CAPTION= <scene name='initialview01'>1kq4</scene>, resolution 2.25&Aring;
+The line below this paragraph, containing "STRUCTURE_1kq4", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= TM0449 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])
+-->
-|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK00847 thyX]</span>
+{{STRUCTURE_1kq4|  PDB=1kq4  |  SCENE=  }}
-|RELATEDENTRY=[[1kq3|1KQ3]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kq4 OCA], [http://www.ebi.ac.uk/pdbsum/1kq4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kq4 RCSB]</span>
-}}
 '''Crystal Structure of Thy1-Complementing Protein (TM0449) from Thermotoga maritima at 2.25 A Resolution'''
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 [[Category: Miller, M D.]]
 [[Category: Wilson, I A.]]
-[[Category: flavin-adenine dinucleotide]]
+[[Category: Flavin-adenine dinucleotide]]
-[[Category: jcsg]]
+[[Category: Jcsg]]
-[[Category: joint center for structural genomic]]
+[[Category: Joint center for structural genomic]]
-[[Category: protein structure initiative]]
+[[Category: Protein structure initiative]]
-[[Category: psi]]
+[[Category: Psi]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: thy1-complementing protein from thermotoga maritima]]
+[[Category: Thy1-complementing protein from thermotoga maritima]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 23:02:07 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:52:00 2008''