1kq1: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1kq1.gif|left|200px]] | [[Image:1kq1.gif|left|200px]] | ||
<!-- | |||
The line below this paragraph, containing "STRUCTURE_1kq1", creates the "Structure Box" on the page. | |||
You may change the PDB parameter (which sets the PDB file loaded into the applet) | |||
or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |||
| | or leave the SCENE parameter empty for the default display. | ||
| | --> | ||
{{STRUCTURE_1kq1| PDB=1kq1 | SCENE= }} | |||
}} | |||
'''1.55 A Crystal structure of the pleiotropic translational regulator, Hfq''' | '''1.55 A Crystal structure of the pleiotropic translational regulator, Hfq''' | ||
| Line 30: | Line 27: | ||
[[Category: Schumacher, M A.]] | [[Category: Schumacher, M A.]] | ||
[[Category: Valentin-Hansen, P.]] | [[Category: Valentin-Hansen, P.]] | ||
[[Category: | [[Category: Hexamer]] | ||
[[Category: | [[Category: Hfq]] | ||
[[Category: | [[Category: Rna binding protein]] | ||
[[Category: | [[Category: Sm motif]] | ||
[[Category: | [[Category: Translational regulator]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 23:01:53 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 23:01, 2 May 2008
1.55 A Crystal structure of the pleiotropic translational regulator, Hfq
OverviewOverview
In prokaryotes, Hfq regulates translation by modulating the structure of numerous RNA molecules by binding preferentially to A/U-rich sequences. To elucidate the mechanisms of target recognition and translation regulation by Hfq, we determined the crystal structures of the Staphylococcus aureus Hfq and an Hfq-RNA complex to 1.55 and 2.71 A resolution, respectively. The structures reveal that Hfq possesses the Sm-fold previously observed only in eukaryotes and archaea. However, unlike these heptameric Sm proteins, Hfq forms a homo-hexameric ring. The Hfq-RNA structure reveals that the single-stranded hepta-oligoribonucleotide binds in a circular conformation around a central basic cleft, whereby Tyr42 residues from adjacent subunits stack with six of the bases, and Gln8, outside the Sm motif, provides key protein-base contacts. Such binding suggests a mechanism for Hfq function.
About this StructureAbout this Structure
1KQ1 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
ReferenceReference
Structures of the pleiotropic translational regulator Hfq and an Hfq-RNA complex: a bacterial Sm-like protein., Schumacher MA, Pearson RF, Moller T, Valentin-Hansen P, Brennan RG, EMBO J. 2002 Jul 1;21(13):3546-56. PMID:12093755 Page seeded by OCA on Fri May 2 23:01:53 2008