1ko3: Difference between revisions

Revision as of 22:58, 2 May 2008

VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa with Cys221 reduced

OverviewOverview

The crystal structures of the universally widespread metallo-beta-lactamase (MBL) Verona integron-encoded MBL (VIM)-2 from Pseudomonas aeruginosa have been solved in their native form as well as in an unexpected oxidised form. This carbapenem-hydrolysing enzyme belongs to the so-called B1 subfamily of MBLs and shares the folding of alpha beta/beta alpha sandwich, consisting of a core of beta-sheet surrounded by alpha-helices. Surprisingly, it showed a high tendency to be strongly oxidised at the catalytic cysteine located in the Cys site, Cys221, which, in the oxidised structure, becomes a cysteinesulfonic residue. Its native structure was obtained only in the presence of Tris(2-carboxyethyl)phosphine. This oxidation might be a consequence of a lower affinity for the second Zn located in the Cys site that would also explain the observed susceptibility of VIM-2 to chelating agents. This modification, if present in nature, might play a role in catalytic down-regulation. Comparison between native and oxidised VIM-2 and a predicted model of VIM-1 (which shows one residue different in the Cys site compared with VIM-2) is performed to explain the different activities and antibiotic specificities.

About this StructureAbout this Structure

1KO3 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

ReferenceReference

The three-dimensional structure of VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa in its reduced and oxidised form., Garcia-Saez I, Docquier JD, Rossolini GM, Dideberg O, J Mol Biol. 2008 Jan 18;375(3):604-11. Epub 2007 Nov 13. PMID:18061205 Page seeded by OCA on Fri May 2 22:58:13 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ko3.gif|left|200px]]
- {{Structure
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= blaVIM-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
+-->
-|DOMAIN=
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-|RELATEDENTRY=[[1ko2|1KO2]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ko3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ko3 OCA], [http://www.ebi.ac.uk/pdbsum/1ko3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ko3 RCSB]</span>
-}}
 '''VIM-2, a Zn-beta-lactamase from Pseudomonas aeruginosa with Cys221 reduced'''
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 [[Category: Garcia-Saez, I.]]
 [[Category: Rossolini, G M.]]
-[[Category: alpha-beta/beta-alpha fold]]
+[[Category: Alpha-beta/beta-alpha fold]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:58:13 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:51:12 2008''