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'''Crystal structure of allophycocyanin''' | '''Crystal structure of allophycocyanin''' | ||
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[[Category: Liu, J Y.]] | [[Category: Liu, J Y.]] | ||
[[Category: Zhang, J P.]] | [[Category: Zhang, J P.]] | ||
[[Category: | [[Category: Helix-turn-helix]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 22:56:03 2008'' | |||
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Revision as of 22:56, 2 May 2008
Crystal structure of allophycocyanin
OverviewOverview
The crystal structure of allophycocyanin from red algae Porphyra yezoensis (APC-PY) at 2.2-A resolution has been determined by the molecular replacement method. The crystal belongs to space group R32 with cell parameters a = b = 105.3 A, c = 189.4 A, alpha = beta = 90 degrees, gamma = 120 degrees. After several cycles of refinement using program X-PLOR and model building based on the electron density map, the crystallographic R-factor converged to 19.3% (R-free factor is 26.9%) in the range of 10.0 to 2.2 A. The r.m.s. deviations of bond length and angles are 0.015 A and 2.9 degrees, respectively. In the crystal, two APC-PY trimers associate face to face into a hexamer. The assembly of two trimers within the hexamer is similar to that of C-phycocyanin (C-PC) and R-phycoerythrin (R-PE) hexamers, but the assembly tightness of the two trimers to the hexamer is not so high as that in C-PC and R-PE hexamers. The chromophore-protein interactions and possible pathway of energy transfer were discussed. Phycocyanobilin 1alpha84 of APC-PY forms 5 hydrogen bonds with 3 residues in subunit 2beta of another monomer. In R-PE and C-PC, chromophore 1alpha84 only forms 1 hydrogen bond with 2beta77 residue in subunit 2beta. This result may support and explain great spectrum difference exists between APC trimer and monomer.
About this StructureAbout this Structure
1KN1 is a Protein complex structure of sequences from Porphyra yezoensis. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of allophycocyanin from red algae Porphyra yezoensis at 2.2-A resolution., Liu JY, Jiang T, Zhang JP, Liang DC, J Biol Chem. 1999 Jun 11;274(24):16945-52. PMID:10358042 Page seeded by OCA on Fri May 2 22:56:03 2008