1kmq: Difference between revisions

Revision as of 22:55, 2 May 2008

Crystal Structure of a Constitutively Activated RhoA Mutant (Q63L)

OverviewOverview

Mutants of the small G protein RhoA that are deficient in GTPase activity and thereby exhibit constitutive molecular signaling activity are commonly used to discover its cellular functions. In particular, two such mutants, Gly14-->Val (G14V) and Gln63-->Leu (Q63L), are often used interchangeably for such studies. However, while their in vitro rates of GTP hydrolysis are very similar, differences are observed in their other functional properties. The structure of G14V-RhoA is known; in order to assess whether structural variations are responsible for functional differences, the crystal structure of a Q63L-RhoA bound to the GTP-analog 5'-guanylylimidodiphosphate (GMPPNP) was determined at 1.5 A resolution. Overall, the structure is very similar to that of G14V-RhoA, but the significantly higher resolution data permit an improved basis for structural analysis and comparison. The data support the notion that differences observed between the mutants in vivo are likely to arise from altered affinities for RhoGDI and not from direct structural differences.

About this StructureAbout this Structure

1KMQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a constitutively activated RhoA mutant (Q63L) at 1.55 A resolution., Longenecker K, Read P, Lin SK, Somlyo AP, Nakamoto RK, Derewenda ZS, Acta Crystallogr D Biol Crystallogr. 2003 May;59(Pt 5):876-80. Epub 2003, Apr 25. PMID:12777804 Page seeded by OCA on Fri May 2 22:55:27 2008

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OCA

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 '''Crystal Structure of a Constitutively Activated RhoA Mutant (Q63L)'''
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-[[Category: constitutive mutant]]
+[[Category: Constitutive mutant]]
-[[Category: gtp-analog]]
+[[Category: Gtp-analog]]
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+[[Category: Gtpase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:55:27 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:50:45 2008''