1kln: Difference between revisions

Revision as of 22:53, 2 May 2008

DNA POLYMERASE I KLENOW FRAGMENT (E.C.2.7.7.7) MUTANT/DNA COMPLEX

OverviewOverview

Klenow fragment of Escherichia coli DNA polymerase I, which was cocrystallized with duplex DNA, positioned 11 base pairs of DNA in a groove that lies at right angles to the cleft that contains the polymerase active site and is adjacent to the 3' to 5' exonuclease domain. When the fragment bound DNA, a region previously referred to as the "disordered domain" became more ordered and moved along with two helices toward the 3' to 5' exonuclease domain to form the binding groove. A single-stranded, 3' extension of three nucleotides bound to the 3' to 5' exonuclease active site. Although this cocrystal structure appears to be an editing complex, it suggests that the primer strand approaches the catalytic site of the polymerase from the direction of the 3' to 5' exonuclease domain and that the duplex DNA product may bend to enter the cleft that contains the polymerase catalytic site.

About this StructureAbout this Structure

1KLN is a Single protein structure of sequence from Escherichia coli. The following page contains interesting information on the relation of 1KLN with [DNA Polymerase]. Full crystallographic information is available from OCA.

ReferenceReference

Structure of DNA polymerase I Klenow fragment bound to duplex DNA., Beese LS, Derbyshire V, Steitz TA, Science. 1993 Apr 16;260(5106):352-5. PMID:8469987 Page seeded by OCA on Fri May 2 22:53:28 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1kln.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1kln |SIZE=350|CAPTION= <scene name='initialview01'>1kln</scene>, resolution 3.200&Aring;
+The line below this paragraph, containing "STRUCTURE_1kln", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1kln|  PDB=1kln  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kln FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kln OCA], [http://www.ebi.ac.uk/pdbsum/1kln PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kln RCSB]</span>
-}}
 '''DNA POLYMERASE I KLENOW FRAGMENT (E.C.2.7.7.7) MUTANT/DNA COMPLEX'''
@@ Line 29: / Line 26: @@
 [[Category: Derbyshire, V.]]
 [[Category: T A., Steitz..]]
-[[Category: double helix]]
+[[Category: Double helix]]
-[[Category: protein-dna complex]]
+[[Category: Protein-dna complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:53:28 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:50:15 2008''