1kjf: Difference between revisions

Revision as of 22:48, 2 May 2008

SUBSTRATE SHAPE DETERMINES SPECIFICITY OF RECOGNITION RECOGNITION FOR HIV-1 PROTEASE: ANALYSIS OF CRYSTAL STRUCTURES OF SIX SUBSTRATE COMPLEXES

OverviewOverview

The homodimeric HIV-1 protease is the target of some of the most effective antiviral AIDS therapy, as it facilitates viral maturation by cleaving ten asymmetric and nonhomologous sequences in the Gag and Pol polyproteins. Since the specificity of this enzyme is not easily determined from the sequences of these cleavage sites alone, we solved the crystal structures of complexes of an inactive variant (D25N) of HIV-1 protease with six peptides that correspond to the natural substrate cleavage sites. When the protease binds to its substrate and buries nearly 1000 A2 of surface area, the symmetry of the protease is broken, yet most internal hydrogen bonds and waters are conserved. However, no substrate side chain hydrogen bond is conserved. Specificity of HIV-1 protease appears to be determined by an asymmetric shape rather than a particular amino acid sequence.

About this StructureAbout this Structure

1KJF is a Protein complex structure of sequences from Human immunodeficiency virus 1. Full crystallographic information is available from OCA.

ReferenceReference

Substrate shape determines specificity of recognition for HIV-1 protease: analysis of crystal structures of six substrate complexes., Prabu-Jeyabalan M, Nalivaika E, Schiffer CA, Structure. 2002 Mar;10(3):369-81. PMID:12005435 Page seeded by OCA on Fri May 2 22:48:53 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1kjf.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1kjf |SIZE=350|CAPTION= <scene name='initialview01'>1kjf</scene>, resolution 2.00&Aring;
+The line below this paragraph, containing "STRUCTURE_1kjf", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/HIV-1_retropepsin HIV-1 retropepsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.16 3.4.23.16] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1])
+-->
-|DOMAIN=
+{{STRUCTURE_1kjf|  PDB=1kjf  |  SCENE=  }}
-|RELATEDENTRY=[[1f7a|1F7A]], [[1kj4|1KJ4]], [[1kj7|1KJ7]], [[1kjg|1KJG]], [[1kjh|1KJH]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1kjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kjf OCA], [http://www.ebi.ac.uk/pdbsum/1kjf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1kjf RCSB]</span>
-}}
 '''SUBSTRATE SHAPE DETERMINES SPECIFICITY OF RECOGNITION RECOGNITION FOR HIV-1 PROTEASE: ANALYSIS OF CRYSTAL STRUCTURES OF SIX SUBSTRATE COMPLEXES'''
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 [[Category: Protein complex]]
 [[Category: Schiffer, C A.]]
-[[Category: p1-p6]]
+[[Category: P1-p6]]
-[[Category: substrate recognition]]
+[[Category: Substrate recognition]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:48:53 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:49:23 2008''