Sandbox 122: Difference between revisions
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#General sequence for the PY-NLS is either a hydrophobic or base at the motif at N-terminus and R-X2-5- P-Y motif at the C-terminus | #General sequence for the PY-NLS is either a hydrophobic or base at the motif at N-terminus and R-X2-5- P-Y motif at the C-terminus | ||
The PY-NLS of 4OO6 contains a basic rather than hydrophobic N-terminal motif. Basic interactions at the N-terminal motif of the PY-NLS include: Arg92, Arg94, and Arg96 of the NLS with Glu588 and Glu496 of Kapβ2. Interactions of the C-terminal R-X2-5- P-Y motif of the NLS include: Pro98 and Tyr99 of the NLS with Ala381, Ala42, Ile456, and Trp459 of Kapβ2. | The PY-NLS of 4OO6 contains a basic rather than hydrophobic N-terminal motif. | ||
<scene name='37/372723/Label_nls_arg_glu_interaction/2'>Basic interactions at the N-terminal </scene> motif of the PY-NLS include: Arg92, Arg94, and Arg96 of the NLS with Glu588 and Glu496 of Kapβ2. Interactions of the C-terminal R-X2-5- P-Y motif of the NLS include: Pro98 and Tyr99 of the NLS with Ala381, Ala42, Ile456, and Trp459 of Kapβ2. | |||
Upon binding Kapβ2, the NLS structure conforms to and makes contact with the internal surface of Kapβ2 C-terminal arch | Upon binding Kapβ2, the NLS structure conforms to and makes contact with the internal surface of Kapβ2 C-terminal arch | ||