Sandbox 130: Difference between revisions
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(ii)PY-NLS has an overall positive charge allowing for electrostatic compatibility with Kapβ2. | (ii)PY-NLS has an overall positive charge allowing for electrostatic compatibility with Kapβ2. | ||
(iii)General sequence for the PY-NLS is either a hydrophobic or basic motif at the N-terminus and a R-X P-Y motif at the C-terminus. | (iii)General sequence for the PY-NLS is either a hydrophobic or basic motif at the N-terminus and a R-X P-Y motif at the C-terminus. | ||
The PY-NLS of 2H4M contains a hydrophobic rather than a basic N-terminus motif. Hydrophobic interactions at the N-terminal motif of the PY-NLS include: Phe273, Gly274, Pro275, Met276 of the NLS with Trp730 and Ile773 of Kapβ2. Interactions of the C-terminal R-X P-Y motif of the NLS include: Arg284 of the NLS with Glu509 and Asp543 of Kapβ2: Pro288 and Tyr289 of the NLS with Ala380, Ala381, Leu419, Ile457, Tro460 and Arg464 of Kapβ2. | |||
Upon binding Kapβ2, the NLS gains structure conforms to and makes contacts with the internal surface of the Kapβ2 C-terminal arch. | |||