Sandbox 130: Difference between revisions
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The 3D structure of the Kapβ2, highlighed in cornflowerblue, shows one of the 20 [https://en.wikipedia.org/wiki/HEAT_repeat_domain HEAT repeats] in silver <scene name='37/372730/4oo6_heatrep_hr3/2'>HR3</scene>. | The 3D structure of the Kapβ2, highlighed in cornflowerblue, shows one of the 20 [https://en.wikipedia.org/wiki/HEAT_repeat_domain HEAT repeats] in silver <scene name='37/372730/4oo6_heatrep_hr3/2'>HR3</scene>. | ||
The NLS located on Kapβ2 cargos are named the PY-NLS and they bind to the C-terminal arch of Kapβ2. The electrostatic potential of the internal surface of Kapβ2 superhelix at the C-terminal arch is negative. | |||
Recognition of the PY-NLS by Kapβ2 fallows certain guidelines: | |||
(i)PY-NLS, when not bound to Kapβ2, lacks secondary structures. | |||
(ii)PY-NLS has an overall positive charge allowing for electrostatic compatibility with Kapβ2. | |||
(iii)General sequence for the PY-NLS is either a hydrophobic or basic motif at the N-terminus and a R-X P-Y motif at the C-terminus. | |||