1k8m: Difference between revisions

Revision as of 22:26, 2 May 2008

Solution Structure of the Lipoic Acid-Bearing Domain of the E2 component of Human, Mitochondrial Branched-Chain alpha-Ketoacid Dehydrogenase

OverviewOverview

The lipoyl-bearing domain (LBD) of the transacylase (E2) subunit of the branched-chain alpha-keto acid dehydrogenase complex plays a central role in substrate channeling in this mitochondrial multienzyme complex. We have employed multidimensional heteronuclear NMR techniques to determine the structure and dynamics of the LBD of the human branched-chain alpha-keto acid dehydrogenase complex (hbLBD). Similar to LBD from other members of the alpha-keto acid dehydrogenase family, the solution structure of hbLBD is a flattened beta-barrel formed by two four-stranded antiparallel beta-sheets. The lipoyl Lys(44) residue resides at the tip of a beta-hairpin comprising a sharp type I beta-turn and the two connecting beta-strands 4 and 5. A prominent V-shaped groove formed by a surface loop, L1, connecting beta 1- and beta 2-strands and the lipoyl lysine beta-hairpin constitutes the functional pocket. We further applied reduced spectral density functions formalism to extract dynamic information of hbLBD from (15)N-T(1), (15)N-T(2), and ((1)H-(15)N) nuclear Overhauser effect data obtained at 600 MHz. The results showed that residues surrounding the lipoyl lysine region comprising the L1 loop and the Lys(44) beta-turn are highly flexible, whereas beta-sheet S1 appears to display a slow conformational exchange process.

About this StructureAbout this Structure

1K8M is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Solution structure and dynamics of the lipoic acid-bearing domain of human mitochondrial branched-chain alpha-keto acid dehydrogenase complex., Chang CF, Chou HT, Chuang JL, Chuang DT, Huang TH, J Biol Chem. 2002 May 3;277(18):15865-73. Epub 2002 Feb 11. PMID:11839747 Page seeded by OCA on Fri May 2 22:26:20 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1k8m.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1k8m |SIZE=350|CAPTION= <scene name='initialview01'>1k8m</scene>
+The line below this paragraph, containing "STRUCTURE_1k8m", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1k8m|  PDB=1k8m  |  SCENE=  }}
-|RELATEDENTRY=[[1k8o|1K8O]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k8m OCA], [http://www.ebi.ac.uk/pdbsum/1k8m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k8m RCSB]</span>
-}}
 '''Solution Structure of the Lipoic Acid-Bearing Domain of the E2 component of Human, Mitochondrial Branched-Chain alpha-Ketoacid Dehydrogenase'''
@@ Line 30: / Line 27: @@
 [[Category: Chuang, J L.]]
 [[Category: Huang, T h.]]
-[[Category: average structure]]
+[[Category: Average structure]]
-[[Category: experimental nmr data]]
+[[Category: Experimental nmr data]]
-[[Category: human bckd]]
+[[Category: Human bckd]]
-[[Category: lipoyl acid bearing]]
+[[Category: Lipoyl acid bearing]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:26:20 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:44:54 2008''