5j4m: Difference between revisions
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==Crystal structure of shaft pilin SpaA from Lactobacillus rhamnosus GG - E269A/D295N double mutant== | ==Crystal structure of shaft pilin SpaA from Lactobacillus rhamnosus GG - E269A/D295N double mutant== | ||
<StructureSection load='5j4m' size='340' side='right' caption='[[5j4m]], [[Resolution|resolution]] 1.99Å' scene=''> | <StructureSection load='5j4m' size='340' side='right'caption='[[5j4m]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5j4m]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4M OCA]. For a <b>guided tour on the structure components</b> use [http:// | <table><tr><td colspan='2'>[[5j4m]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacrg Lacrg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J4M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5J4M FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f44|5f44]], [[5faa|5faa]], [[5fgs|5fgs]], [[5fgr|5fgr]], [[5fie|5fie]], [[5hbb|5hbb]], [[5hdl|5hdl]], [[5hts|5hts]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5f44|5f44]], [[5faa|5faa]], [[5fgs|5fgs]], [[5fgr|5fgr]], [[5fie|5fie]], [[5hbb|5hbb]], [[5hdl|5hdl]], [[5hts|5hts]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LRHM_0426 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=568703 LACRG])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5j4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j4m OCA], [http://pdbe.org/5j4m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5j4m RCSB], [http://www.ebi.ac.uk/pdbsum/5j4m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5j4m ProSAT]</span></td></tr> | |||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Lacrg]] | |||
[[Category: Large Structures]] | |||
[[Category: Chaurasia, P]] | [[Category: Chaurasia, P]] | ||
[[Category: Krishnan, V]] | [[Category: Krishnan, V]] | ||
Revision as of 10:29, 10 June 2020
Crystal structure of shaft pilin SpaA from Lactobacillus rhamnosus GG - E269A/D295N double mutantCrystal structure of shaft pilin SpaA from Lactobacillus rhamnosus GG - E269A/D295N double mutant
Structural highlights
Publication Abstract from PubMedThus far, all solved structures of pilin-proteins comprising sortase-assembled pili are from pathogenic genera and species. Here, we present the first crystal structure of a pilin subunit (SpaA) from a non-pathogen host (Lactobacillus rhamnosus GG). SpaA consists of two tandem CnaB-type domains, each with an isopeptide bond and E-box motif. Intriguingly, while the isopeptide bond in the N-terminal domain forms between lysine and asparagine, the one in the C-terminal domain atypically involves aspartate. We also solved crystal structures of mutant proteins where residues implicated in forming isopeptide bonds were replaced. Expectedly, the E-box-substituted E139A mutant lacks an isopeptide bond in the N-terminal domain. However, the C-terminal E269A substitution gave two structures; one of both domains with their isopeptide bonds present, and another of only the N-terminal domain, but with an unformed isopeptide bond and significant conformational changes. This latter crystal structure has never been observed for any other Gram-positive pilin. Notably, the C-terminal isopeptide bond still forms in D295N-substituted SpaA, irrespective of E269 being present or absent. Although E-box mutations affect SpaA proteolytic and thermal stability, a cumulative effect perturbing normal pilus polymerization was unobserved. A model showing the polymerized arrangement of SpaA within the SpaCBA pilus is proposed. New insights about pilus formation in gut-adapted Lactobacillus rhamnosus GG from the crystal structure of the SpaA backbone-pilin subunit.,Chaurasia P, Pratap S, von Ossowski I, Palva A, Krishnan V Sci Rep. 2016 Jun 28;6:28664. doi: 10.1038/srep28664. PMID:27349405[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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