1k2a: Difference between revisions

Revision as of 22:12, 2 May 2008

Modified Form of Eosinophil-derived Neurotoxin

OverviewOverview

The crystal structure of a post-translationally modified form of eosinophil-derived neurotoxin (EDN) with four extra residues on its N terminus ((-4)EDN) has been solved and refined at atomic resolution (1 A). Two of the extra residues can be placed unambiguously, while the density corresponding to two others is poor. The modified N terminus appears to influence the position of the catalytically important His129, possibly explaining the diminished catalytic activity of this variant. However, (-4)EDN has been shown to be cytotoxic to a Kaposi's sarcoma tumor cell line and other endothelial cell lines. Analysis of the structure and function suggests that the reason for cytotoxicity is most likely due to cellular recognition by the N-terminal extension, since the intrinsic activity of the enzyme is not sufficient for cytotoxicity and the N-terminal extension does not affect the conformation of EDN.

About this StructureAbout this Structure

1K2A is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities., Chang C, Newton DL, Rybak SM, Wlodawer A, J Mol Biol. 2002 Mar 15;317(1):119-30. PMID:11916383 Page seeded by OCA on Fri May 2 22:12:41 2008

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OCA

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 '''Modified Form of Eosinophil-derived Neurotoxin'''
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 [[Category: Rybak, S M.]]
 [[Category: Wlodawer, A.]]
-[[Category: rnase a folding]]
+[[Category: Rnase a folding]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:12:41 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:42:18 2008''