1k1g: Difference between revisions

Revision as of 22:10, 2 May 2008

STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1

OverviewOverview

During spliceosome assembly, splicing factor 1 (SF1) specifically recognizes the intron branch point sequence (BPS) UACUAAC in the pre-mRNA transcripts. We show that the KH-QUA2 region of SF1 defines an enlarged KH (hn RNP K) fold which is necessary and sufficient for BPS binding. The 3' part of the BPS (UAAC), including the conserved branch point adenosine (underlined), is specifically recognized in a hydrophobic cleft formed by the Gly-Pro-Arg-Gly motif and the variable loop of the KH domain. The QUA2 region recognizes the 5' nucleotides of the BPS (ACU). The branch point adenosine acting as the nucleophile in the first biochemical step of splicing is deeply buried. BPS RNA recognition suggests how SF1 may facilitate subsequent formation of the prespliceosomal complex A.

About this StructureAbout this Structure

1K1G is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for recognition of the intron branch site RNA by splicing factor 1., Liu Z, Luyten I, Bottomley MJ, Messias AC, Houngninou-Molango S, Sprangers R, Zanier K, Kramer A, Sattler M, Science. 2001 Nov 2;294(5544):1098-102. PMID:11691992 Page seeded by OCA on Fri May 2 22:10:52 2008

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OCA

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 [[Image:1k1g.gif|left|200px]]
- {{Structure
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-|PDB= 1k1g |SIZE=350|CAPTION= <scene name='initialview01'>1k1g</scene>
+The line below this paragraph, containing "STRUCTURE_1k1g", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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-|GENE=
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-|DOMAIN=
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-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k1g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k1g OCA], [http://www.ebi.ac.uk/pdbsum/1k1g PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1k1g RCSB]</span>
-}}
 '''STRUCTURAL BASIS FOR RECOGNITION OF THE INTRON BRANCH SITE RNA BY SPLICING FACTOR 1'''
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 [[Category: Sprangers, R.]]
 [[Category: Zanier, K.]]
-[[Category: branch point sequence]]
+[[Category: Branch point sequence]]
-[[Category: complex e]]
+[[Category: Complex e]]
-[[Category: kh domain]]
+[[Category: Kh domain]]
-[[Category: protein/rna recognition]]
+[[Category: Protein/rna recognition]]
-[[Category: qua2 homology]]
+[[Category: Qua2 homology]]
-[[Category: splicing]]
+[[Category: Splicing]]
-[[Category: star protein]]
+[[Category: Star protein]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 22:10:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:41:57 2008''