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'''Structure of cephalosporin acylase in complex with glutarate''' | '''Structure of cephalosporin acylase in complex with glutarate''' | ||
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[[Category: Hol, W G.J.]] | [[Category: Hol, W G.J.]] | ||
[[Category: Kim, Y.]] | [[Category: Kim, Y.]] | ||
[[Category: | [[Category: Cephalosporin acylase]] | ||
[[Category: | [[Category: Glutarate]] | ||
[[Category: | [[Category: Glutaryll-7-aca]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 21:59:40 2008'' | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | |||
Revision as of 21:59, 2 May 2008
Structure of cephalosporin acylase in complex with glutarate
OverviewOverview
BACKGROUND: Semisynthetic cephalosporins are primarily synthesized from 7-aminocephalosporanic acid (7-ACA), which is obtained by environmentally toxic chemical deacylation of cephalosporin C (CPC). Thus, the enzymatic conversion of CPC to 7-ACA by cephalosporin acylase (CA) would be of great interest. However, CAs use glutaryl-7-ACA (GL-7-ACA) as a primary substrate and the enzyme has low turnover rates for CPC. RESULTS: The binary complex structures of CA with GL-7-ACA and glutarate (the side-chain of GL-7-ACA) show extensive interactions between the glutaryl moiety of GL-7-ACA and the seven residues that form the side-chain pocket. These interactions explain why the D-alpha-aminoadipyl side-chain of CPC yields a poorer substrate than GL-7-ACA. CONCLUSIONS: This understanding of the nature of substrate specificity may be useful in the design of an enzyme with an improved performance for the conversion of CPC to 7-ACA. Additionally, the catalytic mechanism of the deacylation reaction was revealed by the ligand bound structures.
About this StructureAbout this Structure
1JW0 is a Protein complex structure of sequences from Brevundimonas diminuta. Full crystallographic information is available from OCA.
ReferenceReference
Structure of cephalosporin acylase in complex with glutaryl-7-aminocephalosporanic acid and glutarate: insight into the basis of its substrate specificity., Kim Y, Hol WG, Chem Biol. 2001 Dec;8(12):1253-64. PMID:11755403 Page seeded by OCA on Fri May 2 21:59:40 2008