1jvx: Difference between revisions
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'''Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal''' | '''Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal''' | ||
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[[Category: Samsonoff, W A.]] | [[Category: Samsonoff, W A.]] | ||
[[Category: Srinivasan, U.]] | [[Category: Srinivasan, U.]] | ||
[[Category: | [[Category: Cross-link]] | ||
[[Category: | [[Category: Disulfide]] | ||
[[Category: | [[Category: Intermolecular]] | ||
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Revision as of 21:59, 2 May 2008
Maltodextrin-binding protein variant D207C/A301GS/P316C cross-linked in crystal
OverviewOverview
Cysteine substitutions were engineered on the surface of maltose binding protein to produce crystine fibers, linear polymers of folded protein formed within a crystal. Disulfide bond formation between adjacent protein molecules within the lattice was monitored by X-ray crystallography. The cross-linked crystals were resistant to dissolution in water or neutral buffer solutions, even though the cross-linking was one-dimensional. However, crystine fibers were observed by transmission electron microscopy to dissociate from the crystals in acidic solutions. Some fibers remained associated as two-dimensional bundles or sheets, with a repeat unit along the fibers consistent with the packing of the individual protein molecules in the crystal. Neutralization of the acidic solutions caused the fibers to re-associate as a solid. Crystine threads were drawn out of this solution. In scanning electron microscopy images, many individual fibers could be seen unwinding from the ends of some threads. Crystine fibers are a new type of biomolecular material with potential applications wherever the use of proteins in a fibrous form is desirable, for example, the incorporation of enzymes into cloth or filtration material.
About this StructureAbout this Structure
1JVX is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Crystine: fibrous biomolecular material from protein crystals cross-linked in a specific geometry., Srinivasan U, Iyer GH, Przybycien TA, Samsonoff WA, Bell JA, Protein Eng. 2002 Nov;15(11):895-902. PMID:12538909 Page seeded by OCA on Fri May 2 21:59:33 2008