1jv4: Difference between revisions

Revision as of 21:57, 2 May 2008

Crystal structure of recombinant major mouse urinary protein (rmup) at 1.75 A resolution

OverviewOverview

Major urinary proteins belong to the lipocalin family and are present in the urine of rodents as an ensemble of isoforms with pheromonal activity. The crystal structure of a recombinant mouse MUP (rMUP) was solved by the molecular-replacement technique and refined to an R factor and R(free) of 20 and 26.5%, respectively, at 1.75 A resolution. The structure was compared with an NMR model and with a crystallographic structure of the wild-type form of the protein. The crystal structures determined in different space groups present significantly smaller conformational differences amongst themselves than in comparison with NMR models. Some, but not all, of the conformational differences between the crystal and solution structures can be explained by the influence of crystallographic contacts. Most of the differences between the NMR and X-ray structures were found in the N-terminus and loop regions. A number of side chains lining the hydrophobic pocket of the molecule are more tightly packed in the NMR structure than in the crystallographic model. Surprisingly, clear and continuous electron density for a ligand was observed inside the hydrophobic pocket of this recombinant protein. Conformation of the ligand modelled inside the density is coherent with the results of recent NMR experiments.

About this StructureAbout this Structure

1JV4 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

ReferenceReference

The X-ray structure of a recombinant major urinary protein at 1.75 A resolution. A comparative study of X-ray and NMR-derived structures., Kuser PR, Franzoni L, Ferrari E, Spisni A, Polikarpov I, Acta Crystallogr D Biol Crystallogr. 2001 Dec;57(Pt 12):1863-9. Epub 2001, Nov 21. PMID:11717500 Page seeded by OCA on Fri May 2 21:57:48 2008

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OCA

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 [[Image:1jv4.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1jv4 |SIZE=350|CAPTION= <scene name='initialview01'>1jv4</scene>, resolution 1.75&Aring;
+The line below this paragraph, containing "STRUCTURE_1jv4", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=TZL:2-(SEC-BUTYL)THIAZOLE'>TZL</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= group 1 major urinary protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
+-->
-|DOMAIN=
+{{STRUCTURE_1jv4|  PDB=1jv4  |  SCENE=  }}
-|RELATEDENTRY=[[1df3|1df3]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jv4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jv4 OCA], [http://www.ebi.ac.uk/pdbsum/1jv4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jv4 RCSB]</span>
-}}
 '''Crystal structure of recombinant major mouse urinary protein (rmup) at 1.75 A resolution'''
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 [[Category: Polikarpov, I.]]
 [[Category: Spisni, A.]]
-[[Category: lipocalin recombinant beta barrel]]
+[[Category: Lipocalin recombinant beta barrel]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:57:48 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:39:26 2008''