3oa7: Difference between revisions
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==Structure of the C-terminal domain of Cnm67, a core component of the spindle pole body of Saccharomyces cerevisiae== | ==Structure of the C-terminal domain of Cnm67, a core component of the spindle pole body of Saccharomyces cerevisiae== | ||
<StructureSection load='3oa7' size='340' side='right' caption='[[3oa7]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='3oa7' size='340' side='right'caption='[[3oa7]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3oa7]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[3oa7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bpph2 Bpph2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OA7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OA7 FirstGlance]. <br> | ||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">7, CNM67, YNL225C, N1264 ([ | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">7, CNM67, YNL225C, N1264 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10756 BPPH2])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oa7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oa7 OCA], [https://pdbe.org/3oa7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oa7 RCSB], [https://www.ebi.ac.uk/pdbsum/3oa7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oa7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Bpph2]] | [[Category: Bpph2]] | ||
[[Category: Large Structures]] | |||
[[Category: Frye, J J]] | [[Category: Frye, J J]] | ||
[[Category: Klenchin, V A]] | [[Category: Klenchin, V A]] | ||
Revision as of 10:19, 12 May 2022
Structure of the C-terminal domain of Cnm67, a core component of the spindle pole body of Saccharomyces cerevisiaeStructure of the C-terminal domain of Cnm67, a core component of the spindle pole body of Saccharomyces cerevisiae
Structural highlights
Publication Abstract from PubMedThe spindle pole body of the budding yeast Saccharomyces cerevisiae has served as a model system for understanding microtubule organizing centers, yet very little is known about the molecular structure of its components. We report here the structure of the C-terminal domain of the core component Cnm67 at 2.3 A resolution. The structure determination was aided by a novel approach to crystallization of proteins containing coiled-coils that utilizes globular domains to stabilize the coiled-coils. This enhances their solubility in Escherichia coli and improves their crystallization. The Cnm67 C-terminal domain (residues Asn-429-Lys-581) exhibits a previously unseen dimeric, interdigitated, all alpha-helical fold. In vivo studies demonstrate that this domain alone is able to localize to the spindle pole body. In addition, the structure reveals a large functionally indispensable positively charged surface patch that is implicated in spindle pole body localization. Finally, the C-terminal eight residues are disordered but are critical for protein folding and structural stability. Structure-function analysis of the C-terminal domain of CNM67, a core component of the Saccharomyces cerevisiae spindle pole body.,Klenchin VA, Frye JJ, Jones MH, Winey M, Rayment I J Biol Chem. 2011 May 20;286(20):18240-50. Epub 2011 Mar 24. PMID:21454609[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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