1jhe: Difference between revisions

Revision as of 21:13, 2 May 2008

LEXA L89P Q92W E152A K156A MUTANT

OverviewOverview

LexA repressor undergoes a self-cleavage reaction. In vivo, this reaction requires an activated form of RecA, but it occurs spontaneously in vitro at high pH. Accordingly, LexA must both allow self-cleavage and yet prevent this reaction in the absence of a stimulus. We have solved the crystal structures of several mutant forms of LexA. Strikingly, two distinct conformations are observed, one compatible with cleavage, and the other in which the cleavage site is approximately 20 A from the catalytic center. Our analysis provides insight into the structural and energetic features that modulate the interconversion between these two forms and hence the rate of the self-cleavage reaction. We suggest RecA activates the self-cleavage of LexA and related proteins through selective stabilization of the cleavable conformation.

About this StructureAbout this Structure

1JHE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of LexA: a conformational switch for regulation of self-cleavage., Luo Y, Pfuetzner RA, Mosimann S, Paetzel M, Frey EA, Cherney M, Kim B, Little JW, Strynadka NC, Cell. 2001 Sep 7;106(5):585-94. PMID:11551506 Page seeded by OCA on Fri May 2 21:13:30 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1jhe.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1jhe |SIZE=350|CAPTION= <scene name='initialview01'>1jhe</scene>, resolution 2.5&Aring;
+The line below this paragraph, containing "STRUCTURE_1jhe", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Repressor_lexA Repressor lexA], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.88 3.4.21.88] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= LexA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+-->
-|DOMAIN=
+{{STRUCTURE_1jhe|  PDB=1jhe  |  SCENE=  }}
-|RELATEDENTRY=[[1jhc|1JHC]], [[1jhf|1JHF]], [[1jhh|1JHH]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jhe OCA], [http://www.ebi.ac.uk/pdbsum/1jhe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jhe RCSB]</span>
-}}
 '''LEXA L89P Q92W E152A K156A MUTANT'''
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 [[Category: Pfuetzner, R A.]]
 [[Category: Strynadka, N C.J.]]
-[[Category: c-terminal]]
+[[Category: C-terminal]]
-[[Category: lexa sos repressor]]
+[[Category: Lexa sos repressor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:13:30 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:33:40 2008''