Phosphoenolpyruvate carboxykinase: Difference between revisions

Revision as of 15:25, 31 August 2016

Function

Phosphoenolpyruvate carboxykinase (PEPCK) catalyzes the conversion of oxaloacetate to phosphoenolpyruvate (PEP) and CO₂. PEPCK is part of the gluconeogenesis pathway^[1]. There are 3 types of PEPCK distinguished by the energy source of the reaction which can be GTP, ATP or diphosphate.

Structural highlights

E. coli GTP-driven PEPCK active site is located in a pocket at the enzyme surface^[2].

GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry 1khf)

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3D Structures of PEPCK3D Structures of PEPCK

Updated on 31-August-2016

ReferencesReferences

↑ Hanson RW, Reshef L. Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression. Annu Rev Biochem. 1997;66:581-611. PMID:9242918 doi:http://dx.doi.org/10.1146/annurev.biochem.66.1.581
↑ Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ. Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site. J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:11851336 doi:http://dx.doi.org/10.1006/jmbi.2001.5364

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Michal Harel, Jaime Prilusky, Alexander Berchansky, Joel L. Sussman

[1] Hanson RW, Reshef L. Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression. Annu Rev Biochem. 1997;66:581-611. PMID:9242918 doi:http://dx.doi.org/10.1146/annurev.biochem.66.1.581

[2] Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ. Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site. J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:11851336 doi:http://dx.doi.org/10.1006/jmbi.2001.5364

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	<StructureSection load='1khf' size='~~350~~' side='right' caption='GTP-driven E. coli PEPCK complex with PEP ~~(stick model)~~, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry [[1khf]])' scene=''>		<StructureSection load='1khf' size='450' side='right' caption='GTP-driven E. coli PEPCK complex with PEP, ethanediol, Na+ (purple) and Mn+2 ions (PDB entry [[1khf]])' scene='54/540171/Cv/1'>

	== Function ==		== Function ==