1jbb: Difference between revisions

Revision as of 21:00, 2 May 2008

Ubiquitin Conjugating Enzyme, Ubc13

OverviewOverview

While the signaling properties of ubiquitin depend on the topology of polyubiquitin chains, little is known concerning the molecular basis of specificity in chain assembly and recognition. UEV/Ubc complexes have been implicated in the assembly of Lys63-linked polyubiquitin chains that act as a novel signal in postreplicative DNA repair and I kappa B alpha kinase activation. The crystal structure of the Mms2/Ubc13 heterodimer shows the active site of Ubc13 at the intersection of two channels that are potential binding sites for the two substrate ubiquitins. Mutations that destabilize the heterodimer interface confer a marked UV sensitivity, providing direct evidence that the intact heterodimer is necessary for DNA repair. Selective mutations in the channels suggest a molecular model for specificity in the assembly of Lys63-linked polyubiquitin signals.

About this StructureAbout this Structure

1JBB is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer., VanDemark AP, Hofmann RM, Tsui C, Pickart CM, Wolberger C, Cell. 2001 Jun 15;105(6):711-20. PMID:11440714 Page seeded by OCA on Fri May 2 21:00:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1jbb.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1jbb |SIZE=350|CAPTION= <scene name='initialview01'>1jbb</scene>, resolution 2.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1jbb", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND=
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= Ubc13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+-->
-|DOMAIN=
+{{STRUCTURE_1jbb|  PDB=1jbb  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jbb OCA], [http://www.ebi.ac.uk/pdbsum/1jbb PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jbb RCSB]</span>
-}}
 '''Ubiquitin Conjugating Enzyme, Ubc13'''
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 [[Category: VanDemark, A P.]]
 [[Category: Wolberger, C.]]
-[[Category: e2]]
+[[Category: E2]]
-[[Category: ubiquitin]]
+[[Category: Ubiquitin]]
-[[Category: ubiquitin-conjugating enzyme]]
+[[Category: Ubiquitin-conjugating enzyme]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 21:00:39 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:31:07 2008''