1j3r: Difference between revisions

Revision as of 20:46, 2 May 2008

Crystal structure of Thermococcus litoralis phosphogrucose isomerase complexed with gluconate-6-phosphate

OverviewOverview

The gene encoding phosphoglucose isomerase was cloned from Thermococcus litoralis, and functionally expressed in Escherichia coli. The purified enzyme, a homodimer of 21.5 kDa subunits, was biochemically characterized. The inhibition constants for four competitive inhibitors were determined. The enzyme contained 1.25 mol Fe and 0.24 mol Zn per dimer. The activity was enhanced by the addition of Fe(2+), but inhibited by Zn(2+) and EDTA. Enzymes with mutations in conserved histidine and glutamate residues in their cupin motifs contained no metals, and showed large decreases in k(cat). The circular dichroism spectra of the mutant enzymes and the wild type enzyme were essentially the same but with slight differences.

About this StructureAbout this Structure

1J3R is a Single protein structure of sequence from Thermococcus litoralis. Full crystallographic information is available from OCA.

ReferenceReference

Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis., Jeong JJ, Fushinobu S, Ito S, Jeon BS, Shoun H, Wakagi T, FEBS Lett. 2003 Jan 30;535(1-3):200-4. PMID:12560104 Page seeded by OCA on Fri May 2 20:46:12 2008

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OCA

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 [[Image:1j3r.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1j3r |SIZE=350|CAPTION= <scene name='initialview01'>1j3r</scene>, resolution 2.18&Aring;
+The line below this paragraph, containing "STRUCTURE_1j3r", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=6PG:6-PHOSPHOGLUCONIC+ACID'>6PG</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1j3r|  PDB=1j3r  |  SCENE=  }}
-|RELATEDENTRY=[[1j3p|1J3P]], [[1j3q|1J3Q]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j3r OCA], [http://www.ebi.ac.uk/pdbsum/1j3r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1j3r RCSB]</span>
-}}
 '''Crystal structure of Thermococcus litoralis phosphogrucose isomerase complexed with gluconate-6-phosphate'''
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 [[Category: Shoun, H.]]
 [[Category: Wakagi, T.]]
-[[Category: archaea]]
+[[Category: Archaea]]
-[[Category: cupin superfamily]]
+[[Category: Cupin superfamily]]
-[[Category: gluconate 6-phosphate]]
+[[Category: Gluconate 6-phosphate]]
-[[Category: iron ion]]
+[[Category: Iron ion]]
-[[Category: phosphoglucose isomerase]]
+[[Category: Phosphoglucose isomerase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:46:12 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:28:28 2008''