1isk: Difference between revisions

Revision as of 20:21, 2 May 2008

3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES

OverviewOverview

The three-dimensional structure of the enzyme 3-oxo-delta5-steroid isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by multidimensional heteronuclear magnetic resonance spectroscopy. The two independently folded monomers pack together by means of extensive hydrophobic and electrostatic interactions. Each monomer comprises three alpha helices and a six-strand mixed beta-pleated sheet arranged to form a deep hydrophobic cavity. Catalytically important residues Tyr14 (general acid) and Asp38 (general base) are located near the bottom of the cavity and positioned as expected from mechanistic hypotheses. An unexpected acid group (Asp99) is also located in the active site adjacent to Tyr14, and kinetic and binding studies of the Asp99 to Ala mutant demonstrate that Asp99 contributes to catalysis by stabilizing the intermediate.

About this StructureAbout this Structure

1ISK is a Single protein structure of sequence from Comamonas testosteroni. Full crystallographic information is available from OCA.

ReferenceReference

Solution structure of 3-oxo-delta5-steroid isomerase., Wu ZR, Ebrahimian S, Zawrotny ME, Thornburg LD, Perez-Alvarado GC, Brothers P, Pollack RM, Summers MF, Science. 1997 Apr 18;276(5311):415-8. PMID:9103200 Page seeded by OCA on Fri May 2 20:21:52 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Steroid_Delta-isomerase Steroid Delta-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.3.1 5.3.3.1] </span>
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 '''3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES'''
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 [[Category: Zawrotny, M E.]]
 [[Category: 3-ketosteroid]]
-[[Category: isomerase]]
+[[Category: Isomerase]]
-[[Category: ksi]]
+[[Category: Ksi]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:21:52 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:24:02 2008''