1is7: Difference between revisions

Revision as of 20:21, 2 May 2008

Crystal structure of rat GTPCHI/GFRP stimulatory complex

OverviewOverview

In the presence of phenylalanine, GTP cyclohydrolase I feedback regulatory protein (GFRP) forms a stimulatory 360-kDa complex with GTP cyclohydrolase I (GTPCHI), which is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin. The crystal structure of the stimulatory complex reveals that the GTPCHI decamer is sandwiched by two GFRP homopentamers. Each GFRP pentamer forms a symmetrical five-membered ring similar to beta-propeller. Five phenylalanine molecules are buried inside each interface between GFRP and GTPCHI, thus enhancing the binding of these proteins. The complex structure suggests that phenylalanine-induced GTPCHI x GFRP complex formation enhances GTPCHI activity by locking the enzyme in the active state.

About this StructureAbout this Structure

1IS7 is a Protein complex structure of sequences from Rattus norvegicus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP., Maita N, Okada K, Hatakeyama K, Hakoshima T, Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1212-7. Epub 2002 Jan 29. PMID:11818540 Page seeded by OCA on Fri May 2 20:21:09 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-}}
 '''Crystal structure of rat GTPCHI/GFRP stimulatory complex'''
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 [[Category: Maita, N.]]
 [[Category: Okada, K.]]
-[[Category: enzyme-regulatory protein complex]]
+[[Category: Enzyme-regulatory protein complex]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:21:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:23:55 2008''