1ir6: Difference between revisions

Revision as of 20:18, 2 May 2008

Crystal structure of exonuclease RecJ bound to manganese

OverviewOverview

RecJ, a 5' to 3' exonuclease specific for single-stranded DNA, functions in DNA repair and recombination systems. We determined the crystal structure of RecJ bound to Mn(2+) ion essential for its activity. RecJ has a novel fold in which two domains are interconnected by a long helix, forming a central groove. Mn(2+) is located on the wall of the groove and is coordinated by conserved residues characteristic of a family of phosphoesterases that includes RecJ proteins. The groove is composed of residues conserved among RecJ proteins and is positively charged. These findings and the narrow width of the groove indicate that the groove binds single- instead of double-stranded DNA.

About this StructureAbout this Structure

1IR6 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of exonuclease RecJ bound to Mn2+ ion suggests how its characteristic motifs are involved in exonuclease activity., Yamagata A, Kakuta Y, Masui R, Fukuyama K, Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5908-12. Epub 2002 Apr 23. PMID:11972066 Page seeded by OCA on Fri May 2 20:18:53 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1ir6.jpg|left|200px]]
- {{Structure
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-}}
 '''Crystal structure of exonuclease RecJ bound to manganese'''
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 [[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
 [[Category: Yamagata, A.]]
-[[Category: dna recombination]]
+[[Category: Dna recombination]]
-[[Category: dna repair]]
+[[Category: Dna repair]]
-[[Category: manganese]]
+[[Category: Manganese]]
-[[Category: nuclease]]
+[[Category: Nuclease]]
-[[Category: riken structural genomics/proteomics initiative]]
+[[Category: Riken structural genomics/proteomics initiative]]
-[[Category: rsgi]]
+[[Category: Rsgi]]
-[[Category: single-stranded dna]]
+[[Category: Single-stranded dna]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: two domains interconnected by alpha-helix]]
+[[Category: Two domains interconnected by alpha-helix]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:18:53 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:23:32 2008''