1ij0: Difference between revisions

Revision as of 20:03, 2 May 2008

Coiled Coil Trimer GCN4-pVLS Ser at Buried D Position

OverviewOverview

Coiled coils, estimated to constitute 3-5% of the encoded residues in most genomes, are characterized by a heptad repeat, (abcdefg)(n), where the buried a and d positions form the interface between multiple alpha-helices. Although generally hydrophobic, a substantial fraction ( approximately 20%) of these a- and d-position residues are polar or charged. We constructed variants of the well-characterized coiled coil GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a or a d position. The stability and oligomeric specificity of each variant were measured, and crystal structures of coiled-coil trimers with threonine or serine at either an a or a d position were determined. The structures show how single polar residues in the interface affect not only local packing, but also overall coiled-coil geometry as seen by changes in the Crick supercoil parameters and core cavity volumes.

About this StructureAbout this Structure

1IJ0 is a Single protein structure. Full crystallographic information is available from OCA.

ReferenceReference

Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:11371197 Page seeded by OCA on Fri May 2 20:03:16 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:1ij0.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1ij0 |SIZE=350|CAPTION= <scene name='initialview01'>1ij0</scene>, resolution 1.86&Aring;
+The line below this paragraph, containing "STRUCTURE_1ij0", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ij0|  PDB=1ij0  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ij0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ij0 OCA], [http://www.ebi.ac.uk/pdbsum/1ij0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ij0 RCSB]</span>
-}}
 '''Coiled Coil Trimer GCN4-pVLS Ser at Buried D Position'''
@@ Line 19: / Line 16: @@
 ==About this Structure==
-IJ0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ0 OCA].
+IJ0 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ0 OCA].
 ==Reference==
@@ Line 27: / Line 24: @@
 [[Category: Kim, P S.]]
 [[Category: Malashkevich, V N.]]
-[[Category: coiled coil trimer]]
+[[Category: Coiled coil trimer]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 20:03:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:20:28 2008''