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The entry | ==Ricin toxin in complex with neutralizing single chain monoclonal antibodies (VHHs)== | ||
<StructureSection load='5e1h' size='340' side='right' caption='[[5e1h]], [[Resolution|resolution]] 2.03Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5e1h]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E1H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E1H FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | |||
[[Category: | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e1h OCA], [http://pdbe.org/5e1h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e1h RCSB], [http://www.ebi.ac.uk/pdbsum/5e1h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e1h ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/RICI_RICCO RICI_RICCO]] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity). | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: RRNA N-glycosylase]] | |||
[[Category: Mantis, N]] | |||
[[Category: Rudolph, M J]] | |||
[[Category: Shoemaker, C]] | |||
[[Category: Vance, D]] | |||
[[Category: Ricin toxin]] | |||
[[Category: Toxin-immune system complex]] | |||
[[Category: Vhh]] | |||
Revision as of 07:13, 4 August 2016
Ricin toxin in complex with neutralizing single chain monoclonal antibodies (VHHs)Ricin toxin in complex with neutralizing single chain monoclonal antibodies (VHHs)
Structural highlights
Function[RICI_RICCO] Ricin is highly toxic to animal cells and to a lesser extent to plant cells. The A chain acts as a glycosidase that removes a specific adenine residue from an exposed loop of the 28S rRNA (A4324 in mammals), leading to rRNA breakage. As this loop is involved in elongation factor binding, modified ribosomes are catalytically inactive and unable to support protein synthesis. The A chain can inactivate a few thousand ribosomes per minute, faster than the cell can make new ones. Therefore a single A chain molecule can kill an animal cell. The B chain binds to beta-D-galactopyranoside moieties on cell surface glycoproteins and glycolipids and facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (Lectin activity). |
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